Culture of Animal Cells: A Manual of Basic Technique, 5th Edition

by R. Ian Freshney (Univ. of Glasgow, Scotland)

• ISBN13: 9780471453291
• ISBN10: 0471453293
• Edition: 5th
• Format: Hardcover
• Copyright: 2005-07-01
• Publisher: Wiley-Liss

Summary

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced. The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes: * An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols * New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering * An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment * Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text * Fully updated references * The clearest, most consistent presentation of step-by-step protocols available * Numerous diagrams, photographs, tables, and charts * Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.

Author Biography

R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

Table of Contents

List of Figures.

List of Color Plates.

Preface.

Abbreviations.

1. Introduction.

2. Training Programs.

3. Biology of Cultured Cells.

4. Laboratory Design and Layout.

5. Equipment.

6. Aseptic Technique.

7. Safety, Bioethics, and Validation.

8. Culture Vessels and Substrates.

9. Defined Media and Supplements.

10. Serum-Free Media.

11. Preparation and Sterilization.

12. Primary Culture.

13. Subculture and Cell Lines.

14. Cloning and Selection.

15. Cell Separation.

16. Characterization.

17. Differentiation.

18. Transformation and Immortalization.

19. Contamination.

20. Cryopreservation.

21. Quantitation.

22. Cytotoxocity.

23. Culture of Specific Cell Types.

24. Culture of Tumor Cells.

25. Organotypic Culture.

26. Scale-Up.

27. Specialized Techniques.

28. Problem Solving.

29. In Conclusion.

Appendix I: Preparation of Reagents.

Appendix II: Sources of Equipment and Materials.

Appendix III: Suppliers and Other Resources.

Appendix IV: Glossary.

Appendix V: General Textbooks and Relevant Journals.

References.

Index.