Current Protocols Essential Laboratory Techniques

by Gallagher, Sean R.; Wiley, Emily A.

• ISBN13: 9780470942413
• ISBN10: 047094241X
• Edition: 2nd
• Format: Paperback
• Copyright: 2012-03-19
• Publisher: Current Protocols

Summary

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Author Biography

Sean R. Gallagher, Ph.D. is the Chief Technology Officer and Director of Product Development for UVP, Inc. (Upland, CA), where he oversees applied technology and product development for specialty light source and life science products. He is on the faculty of the Keck Graduate Institute of Applied Life Sciences and Harvey Mudd College (Claremont, CA) and holds eight patents on technology he developed.  He received his doctorate in Botany from the University of California at Riverside and completed a post-doctoral position at Stanford University.

Emily A. Wiley, Ph.D. is an Associate Professor of Biology in the Joint Science Department of the Claremont-McKenna, Scripps, and Pitzer Colleges, Claremont, CA. She received her doctorate in Molecular Genetics from the University of Washington. She is actively involved in graduate and undergraduate teaching courses and is committed to providing proper training to those students. She has an active research program studying chromatin assembly in Tetrahymena thermophila.

Table of Contents

Foreword

Preface

Contributors List

Conversion FactorsFlow Chart: Application of Techniques

Volume/Weight Measurement

Volume Measurement

Weight Measurement

Concentration Measurement

Spectrophotometry

Quantitation of DNA and RNA with Absorption and Fluorescence Spectroscopy

Reagent Preparation

Theoretical Discussion

Practical Discussion

Recipes In-House

Cell Culture Techniques

Aseptic Technique

Culture of Escherichia coli and Related Bacteria

Sample Preparation

Centrifugation and Cell Fractionation In-House

Purification and Concentration of Nucleic Acids

Chromatography

Overview of Chromatography

Column Chromatography

Electrophoresis

Overview of Electrophoresis

Agarose Gel Electrophoresis

SDS-PAGE

Staining of Gels

Blotting

Overview of Blotting

Nucleic Acid Blotting: Southern and Northern

Protein Blotting: Immunoblotting

Labeling DNA and Probe Preparation

Microscopy

Conventional Light Microscopy

Immunofluorescence Microscopy

Enzymatic Reactions

Working with Enzymes

Overview of PCR

Real-Time PCR

Bioinformatics

Using NCBI Blast

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