Cytochrome P450, Part B

by Abelson; Simon; Johnson; Waterman

• ISBN13: 9780121821739
• ISBN10: 0121821730
• Format: Hardcover
• Copyright: 1996-09-03
• Publisher: Elsevier Science

Summary

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Methods presented for * Heterologous expression of P450s * Enzyme assays * Determination of genotype * Insect P450s * Analysis of P450 structure * Regulation

Table of Contents

CONTRIBUTORS TO VOLUME 272	ix	(6)
PREFACE	xv	(2)
VOLUMES IN SERIES	xvii
Section I. Heterologous Expression of P450s	3	(96)
1. Maximizing Expression of Eukaryotic Cytochrome P450s in Escherichia coli	3	(12)
HENRY J. BARNES
2. Construction of Plasmids and Expression in Escherichia coli of Enzymatically Active Fusion Proteins Containing the Heme-Domain of a P450 Linked to NADPH-P450 Reductase	15	(10)
CHARLES W. FISHER
MANJUNATH S. SHET
RONALD W. ESTABROOK
3. N-Terminal Modifications That Alter P450 Membrane Targeting and Function	25	(10)
STEVEN J. PERNECKY
MINOR J. COON
4. Purification of Functional Recombinant P450s from Bacteria	35	(9)
F. PETER GUENGERICH
MARTHA V. MARTIN
ZUYU GUO
YOUNG-JIM CHUN
5. Application of Electrochemistry for P450-Catalyzed Reactions	44	(7)
RONALD W. ESTABROOK
KEVIN M. FAULKNER
MANJUNATH S. SHET
CHARLES W. FISHER
6. Yeast Expression of Animal and Plant P450s in Optimized Redox Environments	51	(14)
DENIS POMPON
BENEDICTE LOUERAT
ALEXIS BRONINE
PHILIPPE URBAN
7. Generation of the Cytosolic Domain of Microsomal P450 52A3 after High-Level Expression in Saccharomyces cerevisiae	65	(11)
ULRICH SCHELLER
THOMAS JURETZEK
WOLF-HAGEN SCHUNCK
8. Use of Heterologous Expression Systems to Study Autoimmune Drug-Induced Hepatitis	76	(10)
SYLVAINE LECOEUR
JEAN-CHARLES GAUTIER
CLAIRE BELLOC
ALINE GAUFFRE
PHILLIPE H. BEAUNE
9. Recombinant Baculovirus Strategy for Coexpression of Functional Human Cytochrome P450 and P450 Reductase	86	(13)
CAROLINE A. LEE
THOMAS A. KOST
COSETTE SERABJIT-SINGH
Section II. Enzyme Assays	99	(100)
10. In Vitro Assessment of Various Cytochromes P450 and Glucuronosyltransferases Using the Antiarrhythmic Propafenone as a Probe Drug	99	(6)
HEYO K. KROEMER
SIGRID BOTSCH
GEORG HEINKELE
MONIKA SCHICK
11. In Vivo and in Vitro Measurement of CYP2C19 Activity	105	(10)
PETER J. WEDLUND
GRANT R. WILKINSON
12. Chlorzoxazone: An in Vitro and in Vivo Substrate Probe for Liver CYP2E1	115	(9)
DANIELE LUCAS
JEAN-FRANCOIS MENEZ
FRANCOIS BERTHOU
13. Assays for CYP1A2 by Testing in Vivo Metabolism of Caffeine in Humans	124	(8)
BING-KOU TANG
WERNER KALOW
14. Assays of Omeprazole Metabolism as a Substrate Probe for Human CYP Isoforms	132	(7)
DONALD J. BIRKETT
TOMMY ANDERSSON
JOHN O. MINERS
15. Use of Tolbutamide as a Substrate Probe for Human Hepatic Cytochrome P450 2C9	139	(6)
JOHN O. MINERS
DONALD J. BIRKETT
16. Assays of CYP2C8-and CYP3A4-Mediated Metabolism of Taxol in Vivo and in Vitro	145	(7)
THOMAS WALLE
17. Tamoxifen Metabolism by Microsomal Cytochrome P450 and Flavin-Containing Monooxygenase	152	(11)
DAVID KUPFER
SHANGARA S. DEHAL
18. Trimethadione: Metabolism and Assessment of Hepatic Drug-Oxidizing Capacity	163	(6)
EINOSUKE TANAKA
YOSHIHIKO FUNAE
19. Antipyrine, Theophylline, and Hexobarbital as in Vivo P450 Probe Drugs	169	(8)
KEES GROEN
DOUWE D. BREIMER
20. Imipramine: A Model Drug for P450 Research	177	(11)
KIM BROSEN
ERIK SKJELBO
KARIN KRAMER NIELSEN
21. Measurement of Human Liver Microsomal Cytochrome P450 2D6 Activity Using [O-Methyl(14)C]Dextromethorphan as Substrate	186	(13)
A. DAVID RODRIGUES
Section III. Determination of Genotype	199	(36)
22. CYP2D6 Multiallelism	199	(11)
ANN K. DALY
VIDAR M. STEEN
KAREN S. FAIRBROTHER
JEFFREY R. IDLE
23. Genetic Tests Which Identify the Principal Defects in CYP2C19 Responsible for the Polymorphism in Mephenytoin Metabolism	210	(8)
JOYCE A. GOLDSTEIN
JOYCE BLAISDELL
24. Genetic Polymorphism of Human Cytochrome P450 2E1	218	(8)
VESSELA NEDELCHEVA
IRENE PERSSON
MAGNUS INGELMAN-SUNDBERG
25. Identification of Allelic Variants of the Human CYP1A1 Gene	226	(9)
KANAME KAWAJIRI
JUNKO WATANABE
SHIN-ICHI HAYASHI
Section IV. Plant P450s	235	(52)
26. Analysis of Herbicide Metabolism by Monocot Microsomal Cytochrome P450	235	(8)
SZE-MEI CINDY LAU
DANIEL P. O'KEEFE
27. Microsome Preparation from Woody Plant Tissues	243	(7)
JERRY HEFNER
RODNEY CROTEAU
28. Detection, Assay, and Isolation of Allene Oxide Synthase	250	(9)
ALAN R. BRASH
WENCHAO SONG
29. Cinnamic Acid Hydroxylase Activity in Plant Microsomes	259	(9)
FRANCIS DURST
IRENE BENVENISTE
MICHEL SCHALK
DANIELE WERCK-REICHHART
30. Isolation of Plant and Recombinant CYP79	268	(7)
BARBARA ANN HALKIER
OLE SIBBESEN
BIRGER LINDBERG MOLLER
31. Cloning of Novel Cytochrome P450 Gene Sequences via Polymerase Chain Reaction Amplification	275	(12)
TIMOTHY A. HOLTON
DIANE R. LESTER
Section V. Insect P450s	287	(28)
32. Preparation of Microsomes from Insects and Purification of CYP6D1 from House Flies	287	(5)
JEFFREY G. SCOTT
33. Quantification of Ecdysteroid Biosynthesis during Short-Term Organ Culture	292	(12)
TIMOTHY J. SLITER
KOMSUN SUDHIVORASETH
JOHN L. MCCARTHY
34. Sampling P450 Diversity by Cloning Polymerase Chain Reaction Products Obtained with Degenerate Primers	304	(11)
MARK J. SYNDER
JULIE A. SCOTT
JOHN F. ANDERSEN
RENE FEYEREISEN
Section VI. Analysis of P450 Structure	315	(66)
35. Structural Alignments of P450s and Extrapolations to the Unknown	315	(11)
SANDRA E. GRAHAM-LORENCE
JULIAN A. PETERSON
36. Predicting the Rates and Regioselectivity of Reactions Mediated by the P450 Superfamily	326	(10)
JEFFREY P. JONES
KENNETH R. KORZEKWA
37. Substrate Docking Algorithms and the Prediction of Substrate Specificity	336	(11)
JAMES J. DE VOSS
PAUL R. ORTIZ DE MONTELLANO
38. Using Molecular Modeling and Molecular Dynamics Simulation to Predict P450 Oxidation Products	347	(11)
MARK D. PAULSEN
JOHN I. MANCHESTER
RICK L. ORNSTEIN
39. Approaches to Crystallizing P450s	358	(10)
THOMAS L. POULOS
40. Crystallization Studies of NADPH-Cytochrome P450 Reductase	368	(13)
JUNG-JA PARK KIM
DAVID L. ROBERTS
SNEZANA DJORDJEVIC
MIUG WANG
THOMAS M. SHEA
BETTIE SUE SILER MASTERS
Section VII. Regulation	381	(50)
41. Sphingolipid-Dependent Signaling in Regulation of Cytochrome P450 Expression	381	(7)
EDWARD T. MORGAN
MARIANA NIKOLOVAKARAKASHIAN
JIN-QIANG CHEN
ALFRED H. MERRILL, JR.
42. Use of Human Hepatocytes to Study P450 Gene Induction	388	(13)
STEPHEN C. STROM
LIUBOMIR A. PISAROV
KENNETH DORKO
MELISSA T. THOMPSON
JOHN D. SCHUETZ
ERIN G. SCHUETZ
43. Cytochrome P450 mRNA Induction: Quantitation by RNA-Polymerase Chain Reaction Using Capillary Electrophoresis	401	(11)
MICHAEL J. FASCO
CHRISTOPHER TREANOR
LAURENCE KAMINSKY
44. Targeted Disruption of Specific Cytochromes P450 and Xenobiotic Receptor Genes	412	(19)
PEDRO M. FERNANDEZ-SALGUERO
FRANK J. GONZALEZ
AUTHOR INDEX	431	(26)
SUBJECT INDEX	457