Cytokines and Colony Stimulating Factors

by Korholz, Dieter; Kiess, Wieland

• ISBN13: 9781588290359
• ISBN10: 1588290352
• Format: Hardcover
• Copyright: 2003-01-01
• Publisher: Humana Pr Inc

Summary

Hands-on laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Although the protocols require little previous experience, even highly skilled researchers will augment their skills with many novel timesaving techniques.

Table of Contents

Preface	v
Contributors	xi
Part I. Introduction
Primary Immunodeficiencies Caused by Defects of Cytokines and Cytokine Receptors	3	(12)
Volker Wahn
Part II. Detection Assays for Cytokines and Growth Factors
Intracellular Detection of T-Cell Cytokines: Differentiation of TH1 and TH2 Cells	15	(8)
Ursula Banning
Dieter Korholz
Analysis of Cytokine Profiles in Human Skin	23	(6)
Jean Krutmann
Renz Mang
Susanne Grether-Beck
Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry	29	(12)
Christian Schultz
Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells	41	(18)
Kerstin Willmann
Evaluation of the Frequency of Virus-Specific CD8+ T Cells by Cytokine Flow Cytometry	59	(12)
Bodo Hoffmeister
Felix Kiecker
Ingolf Surel
Elham Khatamzas
Volker Schuster
Hans-Dieter Volk
Florian Kern
Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms	71	(24)
Thomas Lehrnbecher
Stephen J. Chanock
Apoptosis Induction by TRAIL	95	(22)
Angelika Eggert
Hauke Sieverts
Henning Walczak
Angiogenic Cytokines: Quantitative and Functional Analysis	117	(20)
Angelika Eggert
John H. Maris
Part III. Protocols on Gene Therapy
Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy: An Overview	137	(16)
Christof M. Kramm
Tim Niehues
Nikolai G. Rainov
Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins	153	(18)
Ciriaco A. Piccirillo
Gerald J. Prud'homme
Interferon-β Gene Therapy for the Treatment of Arthritis	171	(10)
Yuti Chernajovsky
Hanna Dreja
Kostas Triantaphyllopoulos
David Gould
Gene Gun-Based In Vivo Gene Transfer: Application to DNA Vaccination	181	(12)
Tohru Sakai
Kunisuke Himeno
Purification of the Eukaryotic Heat-Shock Proteins Hsp70 and gp96	193	(8)
Arne von Bonin
Solveig H. More
Minka Breloer
Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-α Moiety	201	(12)
Jim Xiang
John R. Gordon
Genetic Engineering of Dendritic Cells by Adenovius-Mediated TNF-α Gene Transfer	213	(14)
Jim Xiang
Josh Wu
Retroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood Lymphocytes	227	(8)
Timothy M. Clay
Michael I. Nishimura
Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus	235	(12)
Glyn D. Palmer
Elvire Gouze
Jean-Noel Gouze
Oliver B. Betz
Christopher H. Evans
Steven C. Ghivizzani
Gene Therapy of X-Linked Severe Combined Immunodeficiency	247	(14)
Salima Hacein-Bey-Abina
Genevieve de Saint Basile
Marina Cavazzana-Calvo
Gene Transfer for Generation of Tumor and Leukemia Vaccines	261	(18)
Dagmar Dilloo
Andree Zibert
Cytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral Vectors	279	(14)
Roberto Furlan
Stefano Pluchino
Peggy C. Marconi
Gianvito Martino
Part IV. Ex Vivo Cell Expansion
In Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative Disease	293	(18)
Thomas Vraetz
Peter D. Emanuel
Charlotte M. Niemeyer
In Vitro Generation of Dendritic Cells from Cord Blood CD34+ Hematopoietic Progenitors Cells	311	(16)
Bruno Canque
Michele Rosenzwajg
Jean Claude Gluckman
Generation of Transgenic T Cells from Human CD34+ Cord Blood Cells	327	(14)
Bruno Verhasselt
Evelien Naessens
Veronique Stove
Ex Vivo Expansion of Umbilical Cord Blood Cells on Feeder Layers	341	(10)
Martin Bornhauser
Ex Vivo Production of Cord Blood CD34+ Derived Myeloid Precursors After Serum-Free Static Culture	351	(12)
Sergio Querol
Ex Vivo Expansion of Hematopoietic Stem Cells	363	(12)
Beatriz Albella
Jose Carlos Segovia
Guillermo Guenechea
Juan Antonio Bueren
Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use	375	(12)
Phil Lefebvre
Isaac Cohen
Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells	387	(12)
Kohichiro Tsuji
Takahiro Ueda
Yasuhiro Ebiara
Part V. Maturation and Differentiation of Dendritic Cells
Differentiation of Human Antigen-Presenting Dendritic Cells from CD34+ Hematopoietic Stem Cells In Vitro	399	(10)
Xin-Sheng Ju
Martin Zenke
Dendritic Cell Development from Mobilized Peripheral Blood CD34+ Cells	409	(8)
Deanne M. R. Lathers
Nicholas Achille
M. Rita I. Young
In Vitro Maturation of Dendritic Cells from Blood Progenitors	417	(10)
Lina Matera
Alessandra Galetto
Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood	427	(10)
Pawel Kalinski
Pedro Vieira
Joost H. N. Schuitemaker
Quan Cai
Martien Kapsenberg
Preparation of Human Dendritic Cells for Tumor Vaccination	437	(26)
Michael R. Shurin
Generation of Leukemic Dendritic Cells from Patients with Acute Myeloid Leukemia	463	(10)
Mohamad Mohty
Beatrice Gaugler
Daniel Olive
Index	473