Cytokines A Practical Approach

by Balkwill, Frances R.

• ISBN13: 9780199635665
• ISBN10: 0199635668
• Edition: 2nd
• Format: Paperback
• Copyright: 1995-11-16
• Publisher: IRL Press

Summary

Cytokines are important mediators of immunity, inflammation, and cell growth and are relevant to many areas of biomedical research. This book, now in its second edition, provides detailed up-to-date practical information for the study of cytokines and their receptors, including guides to many of the most useful current techniques in cell and molecular biology, immunology, and biochemistry. In all it contains 184 protocols, providing a comprehensive resource for researchers in many disciplines of biomedical research. This book will be essential for graduate students, researchers and professionals in immunology, infectious diseases, autoimmunity, oncology and biomedical research.

Table of Contents

Cloning and expression of cytokine genes

Southern blotting of cytokine genes

Northern analysis, ribonuclease protection and in situ analysis of cytokine messenger RNA

Polymerase chain reaction to quantitate cytokine mRNA

Comparative polymerase chain reaction

Receptor binding studies

Measurement of cytokine induction of PC-specific phospholipase C and sphingomyelinases

Two dimensional gel elctrophoresis for purification, sequencing and identification of cytokine-induced proteins in normal and malignant cells

Antiviral activity of cytokines

Assays for human B cell responses to cytokines

T cells and cytokines

The generation and quantitation of cell-mediated toxicity

Assays for chemotaxis

Cytokine regulation of endothelial cells

Assays for haematopoietic growth factors

Assays for macrophage activation by cytokines

Measurement of proliferative, cytolytic and cytostatic activity of cytokines

Production of cytokine transgenic and knockout mice

Development of antibodies to cytokines

Visualizing the production of cytokines and their receptors by single human cells

Quantitative biological assays for individual cytokines

RIA, IRMA and ELISA assays for cytokines and their soluble receptors

Table of Contents provided by Publisher. All Rights Reserved.