Diagnostic Virology Protocols

by Stephenson, John R.; Warnes, Alan

• ISBN13: 9781607618164
• ISBN10: 1607618168
• Edition: 2nd
• Format: Hardcover
• Copyright: 2010-12-04
• Publisher: Humana Pr Inc

Summary

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Table of Contents

Preface	p. v
Contributors	p. xi
Detection of Adenoviruses	p. 1
Alphaviruses	p. 17
Detection of Human Caliciviruses in Fecal Samples by RT-PCR	p. 39
Detection of Dengue Virus	p. 51
Detection of Enteroviruses from Clinical Specimens	p. 65
The Detection of Hepatitis Viruses	p. 79
Molecular Detection of Herpesviruses	p. 101
HIV Drug Resistance Testing	p. 123
Detection, Quantification, and Characterisation of HIV/SIV	p. 133
Simultaneous Molecular Detection and Confirmation of Influenza AH5, with Internal Control	p. 161
Detection of Measles, Mumps, and Rubella Viruses	p. 183
Detection of High-Risk Mucosal Human Papillomavirus DNA in Human Specimens by a Novel and Sensitive Multiplex PCR Method Combined with DNA Microarray	p. 195
The Detection of Parvoviruses	p. 213
Detection and Characterization of Polioviruses	p. 233
Detection of Human-Pathogenic Poxviruses	p. 257
Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genus	p. 279
Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens	p. 309
Rotaviruses	p. 325
Detection of Crimean-Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCR	p. 357
Detection of SARS Coronavirus	p. 369
Detection of West Nile Virus	p. 383
Point of Care Testing: Diagnosis Outside the Virology Laboratory	p. 415
Modelling Emerging Viral Epidemics for Public Health Protection	p. 435
Index	p. 467
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