Diagnostic Virology Protocols

by Stephenson, John R.; Warnes, Alan

• ISBN13: 9780896034792
• ISBN10: 0896034798
• Format: Hardcover
• Copyright: 1998-10-01
• Publisher: Humana Pr Inc

Summary

A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

Table of Contents

Preface	v	(6)
Contributors	xi
1 Rapid Detection of Adenovirus from Fecal Specimens	1	(6)
Tanvir Tabish
Alan Warnes
Stuart Clark
2 Alphaviruses	7	(12)
John T. Roehrig
Teresa M. Brown
Alison J. Johnson
Nick Karabatsos
Denise A. Martin
Carl J. Mitchell
Roger S. Nasci
3 Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR	19	(10)
Xi Jiang
David O. Matson
4 Enteroviruses and Rhinoviruses	29	(22)
Peter Muir
5 Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction	51	(12)
Dennis W. Trent
Gwong-Jen Chang
A. Vance Vorndam
Robert S. Lanciotti
6 Hepatitis Viruses	63	(26)
Girish J. Kotwal
7 Herpesviruses	89	(14)
Mark J. Espy
P. Shawn Mitchell
David H. Persing
Thomas F. Smith
8 Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper	103	(16)
Sharon Cassol
Stanley Read
Bruce G. Weniger
Richard Pilon
Barbara Leung
Theresa Mo
9 PCR for the Detection of Influenza Viruses in Clinical Material	119	(10)
Joanna S. Ellis
David W.G. Brown
10 Lyssaviruses: Special Emphasis on Rabies Virus	129	(14)
Herve Bourhy
11 Methods for Detecting Anti-Measles, Mumps, and Rubella Virus Antibodies	143	(16)
Ilkka Julkunen
Irja Davidkin
Christian Oker-Blom
12 Papillomaviruses	159	(14)
Sandra H. Kirk
David T.Y. Liu
13 Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization	173	(16)
Karen E. Hicks
Stuart Beard
Bernard J. Cohen
Jonathan P. Clewley
14 Polioviruses: Concurrent Serotyping and Intratypic Differentiation of Polioviruses	189	(10)
David J. Wood
15 Poxviruses	199	(14)
Hermann Meyer
Susan L. Ropp
Joseph J. Esposito
16 Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections	213	(10)
G.L. Toms
17 Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis	223	(8)
Lennart Svensson
18 Detection of PCR-Amplified Sandfly Fever Sicilian Virus RNA by Colorimetric Enzymatic Immunoassay	231	(14)
M. Sofi Ibrahim
Connie S. Schmaljohn
19 Time-Resolved Fluorescence	245	(12)
Pekka Halonen
Timo Lovgren
20 Enzyme-Linked Immunosorbent Assays	257	(20)
David M. Kemeny
21 Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology	277	(10)
Joakim Lundeberg
Jacob Odeberg
Mathias Uhlen
22 Robotic Analyzers	287	(14)
Dale R. Pfost
Richard G. Keightley
23 The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction	301	(14)
Peter Wyn-Jones
Jane Sellwood
24 Recombinant Antigens in Viral Diagnosis	315	(16)
John R. Stephenson
Alan Warnes
25 NASBA: A Method for Nucleic Acid Diagnostics	331	(10)
Dianne van Strijp
Pierre van Aarle
26 Oligonucleotides	341	(26)
Stina Syrjanen
Index	367