Doubled Haploid Production in Crop Plants

by Maluszynski, M.; Kasha, K. J.; Forster, B. P.; Szarejko, I.

• ISBN13: 9781402015441
• ISBN10: 1402015445
• Format: Hardcover
• Copyright: 2004-01-01
• Publisher: Kluwer Academic Pub

Summary

This is the first manual presenting a set of protocols for production of doubled haploids (DH) in 22 major crop plant species including 4 tree species. It contains various protocols and approaches of DH production proven for different germplasm of the same species. The protocols describe in detail all steps of DH production - from donor plants growth conditions, through in vitro procedures, media composition and preparation, to regeneration of haploid plants and chromosome doubling methods. The users of this manual will be able to choose among microspore versus anther cultures, wide hybridisation or gynogenesis, the most suitable method for production of DH in particular laboratory conditions for their plant material. Numerous colour illustrations should help in this decision. The user will also find information on organization of a doubled haploid laboratory, basic DH media and on associated simple cytogenetic methods for ploidy level analysis. The practical protocols are supplemented with the list of published DH protocols for other crop plants and separate chapters dealing with major applications of DH in breeding, mutant production, transgenesis, and genetic mapping and genomics. The book was prepared by the Plant Breeding and Genetics Section of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture in close co-operation with the EU program 'COST 851' on 'Gametic cells and molecular breeding for crop improvement'.

Table of Contents

Preface	vii
Abbreviations	ix
Colour Figures	xi
1. Production of doubled haploids in crop plants. An introduction
K.J. Kasha and M. Maluszynski l
2. Protocols for major crops
Barley
2.1. Doubled haploid production in barley using the Hordeum bulbosum (L.) technique
P. Hayes, A. Corey and J. DeNoma	5	(10)
2.2. The Hordeum bulbosum (L.) method
P. Devaux	15	(6)
2.3 Anther culture in barley
C. Jacquard, G. Wojnarowiez and C. Clément	21	(8)
2.4. Barley anther culture
L. Cistué, MP. Valles, B. Echávarri, J.M. Sanz and A. Castillo	29	(6)
2.5. Anther culture for doubled haploid production in barley (Hordeum vulgare L.)
I. Szarejko	35	(8)
2.6. Barley isolated microspore culture protocol
K.J. Kasha, E. Simion, R. Oro and Y.S. Shim	43	(6)
2.7. Barley isolated microspore culture (IMC) method
P.A. Davies	49	(4)
Wheat
2.8. Doubled haploid production in wheat through wide hybridization
M.N. Inagaki	53	(6)
2.9. Protocol of wheat (Triticum eastivum L.) anther culture
J. Pauk, R. Mihály and M. Puolimatka	59	(6)
2.10. Protocol for producing doubled haploid plants from anther culture of wheat (Triticum aestivum L.)
B. Barnabás	65	(6)
2.11. Wheat anther culture
S. Tuvesson, R. von Post and A. Ljungberg	71	(6)
2.12. Haploid wheat isolated microspore culture protocol
K.J. Kasha, E. Simion, M Miner, J. Letarte and T. C. Hu	77	(6)
2.13. Production of doubled haploids in wheat (Triticum aestivum L.) through microspore embryogenesis triggered by inducer chemicals
M.Y. Zheng, W. Liu, Y. Weng, E. Polle and C.F. Konak	83	(12)
Maize
2.14. Isolated microspore culture in maize (Zea mat's L.), production of doubled-haploid via induced androgenesis
M.Y. Zheng, Y. Weng, R. Sahibzada and C.F. Konak	95	(8)
2.15. Anther culture of maize (Zea mat's L.)
B. Barnabás	103	(6)
Rice indica/japanica
2.16. Laboratory protocol for anther culture technique in rice
F.J. Zapata-Arias	109	(8)
Triticale
2.17. Triticale anther culture
S. Tuvesson, R. von Post and A. Ljungberg	117	(6)
2.18. Protocol for anther culture in hexaploid triticale (x Triticosecale Wittm.)
M. Wedzony	123	(6)
2.19. Protocol of triticale (x Triticosecale Wittmack) microspore culture
J. Pauk, R. Mihály, T. Monostore and M. Puolimatka	129	(6)
2.20. Protocol for doubled haploid production in hexaploid triticale (x Triticosecale Wittm.) by crosses with maize
M. Wedzony	135	(6)
Rye
2.21. Protocol for rye anther culture
S. Immonen and T. Tenhola-Roininen	141	(10)
2.22. Microspore culture of rye
S. Pulle and Y.-D. Guo	151	(4)
Oat
2.23. Oat haploids from wide hybridization
H.W. Rines	155	(6)
Durum wheat
2.24. Haploid and doubled haploid production in durum wheat by wide hybridization
P.P. Jauhar	161	(6)
2.25. Haploid and doubled haploid production in durum wheat by anther culture
P.P. Jauhar	167	(6)
Timothy
2.26. Anther culture and isolated microspore culture in timothy
S. Pulli and Y.-D. Guo	173	(6)
Ryegrass and other grasses
2.27. Doubled haploid induction in ryegrass and other grasses
S.B.Andersen	179	(6)
Rapeseed
2.28. Microspore culture in rapeseed (Brassica napus L.)
J.B.M Custers	185	(10)
Broccoli
2.29. Protocol for broccoli microspore culture
J.C. da Silva Dias	195	(10)
Other Brassicas
2.30. Microspore culture of Brassica species
A. Ferrie	205	(12)
2.31. Protocol for microspore culture in Brassica
M. Hansen	217	(6)
Tobacco
2.32. Anther and microspore culture in tobacco
A. Touraev and E. Heberle-Bors	223	(6)
Potato
2.33. Haploid production of potatoes by anther culture
G.C.C. Tai and X.Y. Xiong	229	(6)
2.34. Anther culture through direct embryogenesis in a genetically diverse range of potato (Solanum) species and their interspecific and intergeneric hybrids
V.-M Rokka	235	(6)
2.35. Potato haploid technologies
M.J. De, Maine	241	(8)
Linseed/flax
2.36. Anther culture of linseed (Linum usitatissimum L.)
K. Nichterlein	249	(6)
Sugar beet
2.37. Doubled haploid production of sugar beet (Beta vulgaris L.)
E. Wremerth Weich and M.W. Levall	255	(10)
Asparagus
2.38. Asparagus microspore and anther culture
D.J. Wolyn and B.Nichols	265	(10)
Onion
2.39. In vitro gynogenesis induction and doubled haploid production in onion (Allium cepa L.)
L. Martinez	275	(6)
2.40. Haploid induction in onion via gynogenesis
M. Jakše and B. Bohanec	281	(6)
Apple
2.41. In vitro androgenesis in apple
M. Höfer	287	(6)
Aspen
2.42. Doubled haploid production in poplar
S.B. Andersen	293	(4)
Cork oak
2.43. Oak anther culture
M.A. Bueno and J.A. Manzanera	297	(6)
Citrus
2.44. Haploids and doubled haploids in Citrus spp.
M.A. Germanà	303
3. Published protocols for other crop plant species
M. Maluszynski, K.J. Kasha and I. Szarejko	309	(28)
4. Application of doubled haploid production techniques
4.1. Doubled haploids in breeding
W.T.B. Thomas, B.P. Forster and B. Gertsson	337	(14)
4.2. Doubled haploid mutant production
I. Szarejko	351	(12)
4.3. Barley microspore transformation protocol by biolistic gun
Y.S. Shim and K.J. Kasha	363	(4)
4.4. Doubled haploids in genetic mapping and genomics
B.P. Forster and W.T.B. Thomas	367	(24)
5. Protocols for chromosome counting
5.1. Cytogenetic tests for ploidy level analyses - chromosome counting
J. Maluszynska	391	(6)
5.2. Ploidy determination using flow cytometry
B. Bohanec	397	(8)
6. Major media composition and basic equipment for DH laboratory
6.1. Major media composition
compiled by I. Szarejko	405	(10)
6.2. Basic equipment for maize microspore culture laboratory
compiled by M.Y. Zheng, Y. Weng, R. Sahibzada and C.F. Konak	415	(2)
List of contributors	417