The Encyclopedia of Mass Spectrometry

by Caprioli; Gross

• ISBN13: 9780080438009
• ISBN10: 0080438008
• Edition: 1st
• Format: Hardcover
• Copyright: 2004-09-27
• Publisher: Elsevier Science

Summary

Volume 2: Biological Applications Part AThe focus of Volume 2 is peptides and proteins. The organization emphasizes separation techniques, preparation protocols, and fundamentals of ionic gas-phase species of biological importance. This volume is divided into four sections: (1) experimental approaches and protocols, (2) sequence analysis, (3) other structural analyses, and (4) targeted applications.The first section encompass separation procedures (e.g., 2-D gel electrophoresis), sample preparation (e.g., desalting and enzyme digestion), and instrumentation issues (e.g., high resolving power, molecular-weight determination, protein chips, and quantification). H/D exchange, analysis of membrane proteins, and bioinformatics are included.The next section on sequencing covers high energy and low energy CAD, protein identification, fundamentals of peptide fragmentation, bottom-up and top-down strategies, chemical derivatization, and post-source decay with MALDI. A section on structure analysis includes primary structure determination and issues with studying quaternary structure, protein-protein and protein-ligand complexes, disulfide analysis, phosphopeptides and phosphoproteins, selenoproteins, nitrated proteins, metal ion binding, and oxidized proteins. Additional coverage of methods for studying the biophysics of proteins is provided in Volume 6. The last chapter, Targeted Applications, focuses on neuropeptides, clinical applications, enzyme kinetics, imaging, and single-cell analysis. * Frontier Research on biological, biochemical, and biomedical applications of MS * Focus on Peptides and Proteins * Suitable for new graduate students entering the field

Table of Contents

Chemical modification/damage

Derivatization for Analysis

Desalting/purification/microdialysis

Disulfide Bond Analysis

Enzyme kinetics

Folding/Unfolding

Fragmentation Mechanisms of Ions

Gel Electrophoresis

Glycoproteins

H/D Exchange Methods

High-resolution Measurements by FTMS

High Sensitivity Detection

Hydrolysis, Chemical & Enzymatics

Image Analysis of Proteins in Tissues

Protein Complexes

LC/MS. Membrane proteins

Metal Ion Binding

Molecular Weight Measurements

Neuropeptides

Non-covalent Interactions, Ligand/Protein

Non-covalent Interactions, Protein/Protein

Phosphorylation, sulfation. Proteomics

Quantitation/Proteins. Quaternary Structure

Secondary Structure. Sequence Analysis - Digestion/Cleavage

Sequence Analysis - High Energy MS/MS

Sequence Analysis - Low Energy MS/MS

Sequence Analysis - PSD with MALDI

Single Cell, analysis of, Sweedler

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