#marketplace, .navbar, .container, .container-fluid, #media, #category, #ewards, #careers, .blueHeroSearchContainer, #photoHeroBanner, #etextbooks-page-2015, .slider-arrow, #books-page-2015, #my-account-wrapper-2015 {display:none;} .noscriptmsg p, .noscriptmsg h1 {color: #000;} body {background-color: #ccc !important; font-size: 14px; }

We're sorry, but eCampus.com doesn't work properly without JavaScript.

Either your device does not support JavaScript or you do not have JavaScript enabled.

How to enable JavaScript in your browser.

Need help? Call 1-855-252-4222

Rent More, Save More! Use code: ECRENTAL

5% off 1 book, 7% off 2 books, 10% off 3+ books

Environmental Microbiology

by Pepper; Gerba; Brendecke

ISBN13: 9780125506564
ISBN10: 0125506562
Edition: 2nd
Format: Paperback
Copyright: 12/13/2004
Publisher: Elsevier Science

Your order must be $35 or more to qualify for free economy shipping. Bulk sales, PO's, Marketplace items, eBooks and apparel do not qualify for this offer.
1. Environmental Microbiology > ISBN13: 9780125506564

List Price: $52.95

Summary

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on the soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens--bacteria, viruses, and protozoan parasites--used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material. * New edition incorporates new experiments and the latest techniques * Designed for maximum versatility and ease of use for the student and instructor * Each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Preface

xiii

Basics

xiii

Manual Conventions

xiv

Suggested Soil Types and Tests

SECTION ONE Basic Protocols

(16)

Dilution and Plating of Bacteria and Growth Curves

(8)

Overview

(1)

Theory and Significance

(1)

Procedure

(5)

Tricks of the Trade

(1)

Potential Hazards

(1)

Example Calculation of Mean Generation Time

(1)

Questions and Problems

(1)

Reference

(1)

Soil Moisture Content Determination

(6)

Overview

(1)

Theory and Significance

(2)

Procedure

(1)

Tricks of the Trade

(1)

Potential Hazards

(1)

Example Calculations

(1)

Questions and Problems

(1)

References

(1)

SECTION TWO Examination of Soil Microorganisms Via Microscopic and Cultural Assays

(42)

Contact Slide Assay

(8)

Overview

(1)

Theory and Significance

(2)

Procedure

(2)

Tricks of the Trade

(1)

Potential Hazards

(1)

Questions and Problems

(1)

References

(2)

Filamentous Fungi

(10)

Overview

(1)

Theory and Significance

(3)

Procedure

(2)

Tricks of the Trade

(2)

Potential Hazards

(1)

Calculations

(1)

Questions and Problems

(1)

References

(1)

Bacteria and Actinomycetes

(14)

Overview

(1)

Theory and Significance

(4)

Procedure

(6)

Tricks of the Trade

(1)

Potential Hazards

(1)

Questions and Problems

(1)

References

(2)

Algae: Enumeration by MPN

(8)

Overview

(1)

Theory

(1)

Procedure

(2)

Tricks of the Trade

(1)

Potential Hazards

(1)

Calculations

(3)

Questions and Problems

(1)

References

(1)

SECTION THREE Microbial Transformations and Response to Contaminants

(54)

Oxidation of Sulfur in Soil

(10)

Overview

(1)

Theory

(3)

Procedure

(3)

Tricks of the Trade

(1)

Potential Hazards

(1)

Calculations

(1)

Questions and Problems

(1)

References

(2)

Dehydrogenase Activity of Soils

(6)

Overview

(1)

Theory

(2)

Procedure

(2)

Tricks of the Trade

(1)

Potential Hazards

(1)

Example Calculations

(1)

Questions and Problems

(1)

References

(1)

Nitrification and Denitrification

(8)

Overview

(1)

Theory

(2)

Procedure

(3)

Tricks of the Trade

(1)

Potential Hazards

(1)

Assignment and Questions

(1)

References

(2)

Enrichment and Isolation of Bacteria that Degrade 2,4-Dichlorophenoxyacetic Acid

(6)

Overview

(1)

Theory and Significance

(1)

Procedure

(2)

Tricks of the Trade

(1)

Potential Hazards

(1)

Questions and Problems

(1)

References

(2)

Adaptation of Soil Bacteria to Metals

(4)

Overview

(1)

Theory and Significance

(1)

Procedure

(1)

Tricks of the Trade

(1)

Potential Hazards

(1)

Questions and Problems

(1)

References

(1)

Biodegradation of Phenol Compounds

(4)

Overview

(1)

Theory and Significance

(1)

Procedure

(1)

Potential Hazards

(1)

Calculations

(1)

Questions and Problems

(1)

References

(1)

Assimilable Organic Carbon

(6)

Overview

(1)

Theory and Significance

(1)

Procedure

100

(2)

Tricks of the Trade

102

(1)

Calculations

102

(1)

Questions and Problems

103

(1)

References

103

(2)

Biochemical Oxygen Demand

105

(8)

Overview

105

(1)

Theory and Significance

105

(1)

Procedure

106

(4)

Tricks of the Trade

110

(1)

Potential Hazards

110

(1)

Calculations

111

(1)

Questions and Problems

112

(1)

References

112

(1)

SECTION FOUR Water Microbiology

113

(34)

Bacteriological Examination of Water: The Coliform MPN Test

115

(8)

Overview

115

(1)

Theory and Significance

115

(3)

Procedure

118

(3)

Tricks of the Trade

121

(1)

Calculations

121

(1)

Questions and Problems

122

(1)

Reference

122

(1)

Membrane Filter Technique

123

(6)

Overview

123

(1)

Theory and Significance

123

(1)

Procedure

124

(2)

Tricks of the Trade

126

(1)

Potential Hazards

127

(1)

Calculations

127

(1)

Questions and Problems

127

(1)

Reference

127

(2)

Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms

129

(6)

Overview

129

(1)

Theory and Significance

129

(1)

Procedure

130

(2)

Tricks of the Trade

132

(1)

Potential Hazards

132

(1)

Calculations

132

(1)

Questions and Problems

133

(1)

References

133

(2)

Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces

135

(6)

Overview

135

(1)

Theory and Significance

135

(1)

Procedure

136

(3)

Tricks of the Trade

139

(1)

Questions and Problems

139

(1)

Reference

139

(2)

Detection of Bacteriophages

141

(6)

Overview

141

(1)

Theory and Significance

141

(1)

Procedure

142

(2)

Tricks of the Trade

144

(1)

Potential Hazards

145

(1)

Questions and Problems

145

(1)

References

145

(2)

SECTION FIVE Advanced Topics

147

(40)

Detection of Enteric Viruses in Water

149

(8)

Overview

149

(1)

Theory and Significance

149

(3)

Procedure

152

(2)

Questions and Problems

154

(1)

References

154

(3)

Detection of Waterborne Parasites

157

(6)

Overview

157

(1)

Theory and Significance

157

(4)

Procedure

161

(1)

Questions and Problems

161

(1)

References

162

(1)

Kinetics of Disinfection

163

(6)

Overview

163

(1)

Theory and Significance

163

(1)

Procedure

164

(2)

Tricks of the Trade

166

(1)

Potential Hazards

167

(1)

Calculations

167

(1)

Questions and Problems

167

(1)

References

167

(2)

Aerobiology: Sampling of Airborne Microorganisms

169

(6)

Overview

169

(1)

Theory and Significance

169

(2)

Procedure

171

(2)

Tricks of the Trade

173

(1)

Calculations

173

(1)

Questions and Problems

173

(1)

References

174

(1)

Detection and Identification of Bacteria Via PCR and Subsequent BLAST Analysis of Amplified Sequences

175

(12)

Overview

175

(1)

Theory and Significance

175

(5)

Procedure

180

(4)

Tricks of the Trade

184

(1)

Potential Hazards

184

(1)

Questions and Problems

184

(1)

References

185

(2)

Appendix 1 Preparation of Media and Stains for Each Experiment

187

(10)

Appendix 2 Glossary

197

Supplemental Materials

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.

Rent More, Save More! Use code: ECRENTAL

Rent More, Save More! Use code: ECRENTAL

5% off 1 book, 7% off 2 books, 10% off 3+ books

Environmental Microbiology

Summary

Table of Contents

Supplemental Materials

Rewards Program