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9780125506557

Environmental Microbiology : A Laboratory Manual

by ; ;
  • ISBN13:

    9780125506557

  • ISBN10:

    0125506554

  • Edition: 1st
  • Format: Paperback
  • Copyright: 1995-08-23
  • Publisher: Elsevier Science & Technology

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Summary

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens -- bacteria, viruses, and protozoan parasites -- used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Table of Contents

Preface Basic Protocols Experiment
Dilution and Plating of Bacteria and Growth Curves Experiment
Soil Moisture Content Determination Examination of Soil Microorganisms via Microscopic and Cultural Assays Experiment
Contact Slide Assay Experiment
Filamentous Fungi Experiment
Bacteria and Actinomycetes Experiment
Algae: Enumeration by MPN Microbial Transformations and Response to Contaminants Experiment
Oxidation of Sulfur in Soil Experiment
Dehydrogenase Activity of Soils Experiment
Nitrification and Denitrification Experiment
Enrichment and Isolation of Bacteria that Degrade 2, 4-dichlorophenoxyacetic acid Experiment
Adaptation of Soil Bacteria to Metals Experiment
Biodegradation of Phenol Compounds Experiment
Assimilable Organic Matter Experiment
Biochemical Oxygen Demand Water Microbiology Experiment
Membrane Filter Technique Experiment
Bacteriological Examination of Water: the Coliform MPN Test Experiment
Detection of Bacteriophages Experiment
Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces Experiment
Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms Advanced Topics Experiment
Detection of Enteric Viruses in Water Experiment
Detection of Waterborne Parasites Experiment
Kinetics of Disinfection Experiment
Aerobiology: Sampling of Airborne Microorganisms Experiment
Detection and Identification of Bacteria via PCR and Subsequent BLAST Analysis of Amplified Sequences
Preparation of Media and Stains for Each Experiment
Glossary
Table of Contents provided by Publisher. All Rights Reserved.

Supplemental Materials

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