Environmental Microbiology : A Laboratory Manual

by Pepper; Gerba; Brendecke

• ISBN13: 9780125506557
• ISBN10: 0125506554
• Edition: 1st
• Format: Paperback
• Copyright: 1995-08-23
• Publisher: Elsevier Science & Technology

Summary

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology. The first section includes experiments on soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens -- bacteria, viruses, and protozoan parasites -- used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Table of Contents

Preface Basic Protocols Experiment

Dilution and Plating of Bacteria and Growth Curves Experiment

Soil Moisture Content Determination Examination of Soil Microorganisms via Microscopic and Cultural Assays Experiment

Contact Slide Assay Experiment

Filamentous Fungi Experiment

Bacteria and Actinomycetes Experiment

Algae: Enumeration by MPN Microbial Transformations and Response to Contaminants Experiment

Oxidation of Sulfur in Soil Experiment

Dehydrogenase Activity of Soils Experiment

Nitrification and Denitrification Experiment

Enrichment and Isolation of Bacteria that Degrade 2, 4-dichlorophenoxyacetic acid Experiment

Adaptation of Soil Bacteria to Metals Experiment

Biodegradation of Phenol Compounds Experiment

Assimilable Organic Matter Experiment

Biochemical Oxygen Demand Water Microbiology Experiment

Membrane Filter Technique Experiment

Bacteriological Examination of Water: the Coliform MPN Test Experiment

Detection of Bacteriophages Experiment

Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces Experiment

Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms Advanced Topics Experiment

Detection of Enteric Viruses in Water Experiment

Detection of Waterborne Parasites Experiment

Kinetics of Disinfection Experiment

Aerobiology: Sampling of Airborne Microorganisms Experiment

Detection and Identification of Bacteria via PCR and Subsequent BLAST Analysis of Amplified Sequences

Preparation of Media and Stains for Each Experiment

Glossary

Table of Contents provided by Publisher. All Rights Reserved.