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9781119555988

Equine Reproductive Procedures

by ;
  • ISBN13:

    9781119555988

  • ISBN10:

    1119555981

  • Edition: 2nd
  • Format: Hardcover
  • Copyright: 2021-06-09
  • Publisher: Wiley-Blackwell

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Summary

The second edition of Equine Reproductive Procedures provides equine practitioners, veterinary students, and equine theriogenologists with a fully updated, practical guide to techniques in equine reproductive practice. This easy-to-use resource covers skills-based information with a clinical focus, taking an easy-to-follow step-by-step approach.

The book covers topics related to the reproductive management of horses, including diagnostic techniques and therapeutic procedures for stallions, mares, and foals. Procedures highlighted in the book are supported with clear descriptions and photographs. Readers will also find a list of required supplies for the procedure and a reference list.

  • Provides step-by-step descriptions of techniques related to equine theriogenology
  • Serves as a valuable practice tool
  • Offers 39 new techniques not found in the first edition
  • Includes key updates to existing techniques

 Those studying equine reproduction and specialists in theriogenology will find this an essential ‘how-to’ guide for their practice library.

Author Biography

The editors

John Dascanio, VMD, Diplomate ACT, Diplomate ABVP (Equine) is the senior associate dean at the Texas Tech School of Veterinary Medicine in Amarillo, Texas, USA and was named the 2018 Theriogenologist of the Year by the American College of Theriogenologists.

Patrick McCue, DVM, PhD, Diplomate ACT is the Iron Rose Ranch Professor of Equine Theriogenology at Colorado State University College of Veterinary Medicine and Biomedical Sciences in Fort Collins, Colorado, USA and was named the 2017 Theriogenologist of the Year by the American College of Theriogenologists.

Table of Contents

1 Reproductive Evaluation of the Mare

Patrick M. McCue

2 Teasing

Patrick M. McCue

3 Tail Wrap and Preparation/Washing of the Perineum

John J. Dascanio

4 Placement of a Tail Rope

John J. Dascanio

5 Perineal Conformation Evaluation

John J. Dascanio

6 Removal of a Persistent Hymen

John J. Dascanio

7 Palpation of the Reproductive Tract of the Non-Pregnant Mare

Patrick M. McCue

8 Ultrasound Evaluation of the Non-Pregnant Mare

Patrick M. McCue

9 Prediction of Ovulation

Patrick M. McCue

10 Speculum Examination of the Vagina

John J. Dascanio

11 Digital Examination of the Vagina/Cervix

Sofie Sitters

12 Uterine Culture Collection: Swab/Brush

John J. Dascanio

13 Antimicrobiotic Sensitivity Testing

Jillian Bishop and Patrick M. McCue

14 Microbiology: Microbial Culture

Patrick M. McCue and Jillian Bishop

15 Microbiology: Gram Stain

Jillian Bishop and Patrick M. McCue

16 qPCR Assay for the Diagnosis of Infectious Endometritis

Ryan A. Ferris and Patrick M. McCue

17 Uterine Cytology Collection: Swab/Brush

John J. Dascanio and Ryan A. Ferris

18 Uterine Culture/Cytology: Low Volume Lavage

John J. Dascanio

19 Endometrial Biopsy

Patrick M. McCue

20 Hysteroscopic Examination of the Uterus

Patrick M. McCue

21 Endometrial Cyst Removal

John J. Dascanio

22 Chromosomal Analysis

Terje Raudsepp

23 Endocrinological Examination of the Mare

Patrick M. McCue

24 Laparoscopic Examination of the Uterus and Ovaries

Dean A. Hendrickson

25 Evaluation of the Mammary Gland

Patrick M. McCue

26 Antisperm Antibody Testing

Patrick M. McCue

27 Starch Granule Test for the Evaluation of Oviductal Patency

Sofie Sitters and John J. Dascanio

28 Fluorescent Microspheres Test for Evaluation of Oviductal Patency

Sofie Sitters and John J. Dascanio

29 Oviductal Flush Procedure for the Evaluation of Oviductal Patency

Sofie Sitters and Patrick M. McCue

30 Prostaglandin E2 Treatment for Blocked Oviducts

Patrick M. McCue, Sofie Sitters and Charles F. Scoggin

31 Prostaglandin E1 Treatment for Blocked Oviducts

Patrick M. McCue and Charles F. Scoggin

32 Hysterocoscopic Hydrotubation of the Oviducts

Charles F. Scoggin

33 Transvaginal Ovarian Biopsy

Claire E. Card

34 Uterine Lavage

John J. Dascanio

35 Uterine Infusion

John J. Dascanio

36  Therapeutics for Infectious Endometritis

Ryan A. Ferris

37 Bacterial Biofilm and Endometritis

Ryan A. Ferris

38 Latent Uterine Bacterial Infections.

Morten Rønn Petersen and Anders Miki Bojesen

39 Acupuncture in Mare Reproduction

Kristina Lu and Lauren Javernick

40 Restraint for Breeding

John J. Dascanio

41 Natural Service: Pasture Breeding

Patrick M. McCue

42 Natural Service: In-hand Breeding

Charles F. Scoggin

43 Breeding Stitches

Charles F. Scoggin

44 Reinforcement Breeding

Charles F. Scoggin

45 Breeding with Fresh or Cooled Semen

John J. Dascanio and Patrick M. McCue

46 Insemination Through a Vaginal Speculum

Patrick M. McCue

47 Breeding with Frozen Semen

John J. Dascanio

48 Deep Horn Insemination

John J. Dascanio

49 Hysteroscopic (Low Dose) Insemination

Patrick M. McCue

50 Transvaginal oocyte pickup (OPU)

Jennifer N. Hatzel

51 Packaging and Transport of Oocytes

Jennifer N. Hatzel

52 Intracytoplasmic Sperm Injection (ICSI)

Jennifer N. Hatzel and JoAnne Stokes

53 Caslick Operation or Vulvoplasty

John J. Dascanio

54 Contagious Equine Metritis Testing

John J. Dascanio

55 Management of Seasonal Anestrus: Artificial Photoperiod

Patrick M. McCue

56 Management of Seasonal Anestrus: Hormone Therapy

Patrick M. McCue

57 Hormone Therapy in Cycling Mares

Patrick M. McCue

58 Estrus Suppression

Patrick M. McCue

59 Intra-uterine Devices for Estrus Suppression

Carlos M. Gradil

60 Immunocontraception

Patrick M. McCue

61 Harvesting and Shipping Ovaries for Oocyte Recovery

Patrick M. McCue

62 Embryo Collection

Patrick M. McCue

63 Embryo Evaluation

Patrick M. McCue

64 Embryo Biopsy

Patrick M. McCue

65 Embryo Packaging for Cooled Transport

Patrick M. McCue

66 Embryo Cryopreservation

Patrick M. McCue

67 Non-Surgical Embryo Transfer

Patrick M. McCue

68 Autogenous Transfer of ICSI Derived Embryos

Jennifer N. Hatzel

69 Palpation of the Pregnant Mare Per Rectum

Sofie Sitters

70 Ultrasound Examination of the Pregnant Mare

Patrick M. McCue

71 Speculum Examination of the Pregnant Mare

Sofie Sitters

72 Determination of Equine Fetal Sex 55 – 200 days

Richard D. Holder

73 Assessment of Late Term Fetal Well-being

John J. Dascanio

74 Combined Thickness of the Uterus and Placenta

Patrick M. McCue

75 Placentitis – Diagnosis and Treatment

Justin W. McNaughten and Margo L. Macpherson

76 Doppler Ultrasonography

John J. Dascanio

77 Prediction of Fetal Age

John J. Dascanio

78 Twin Reduction: Manual Technique

John J. Dascanio

79 Twin Reduction: Transvaginal Aspiration

John J. Dascanio

80 Twin Reduction:  Cranio-Cervical Dislocation

Karen Wolfsdorf

81 Twin Reduction: Thoracic Compression

Patrick M. McCue

82 Twin Reduction: Transabdominal Fetal Cardiac Puncture

John J. Dascanio

83 Elective Termination of Pregnancy

John J. Dascanio

84 Abortion Diagnostic Evaluation: Sample Collection in the Field and Submission

Julie T. Cecere and John J. Dascanio

85 Endocrine Evaluation of Pregnancy

Patrick M. McCue

86 Treatment of Vaginal Varicosities

John J. Dascanio

87 Manual Correction of Uterine Torsion

John J. Dascanio

88 Preparation of the Mare for Foaling

John J. Dascanio

89 Assessment of Mammary Gland Secretions to Predict Foaling

John J. Dascanio

90 Assessment of pH of Mammary Gland Secretions to Predict Foaling

Patrick M. McCue

91 Monitoring of Peri-Parturient Mares Using Video and Web Cameras

Ryan A. Ferris

92 Foaling Alert: Vulvar Device

John J. Dascanio

93 Positional Labor Alert Devices

Ryan A. Ferris

94 Induction of Parturition

John J. Dascanio

95 Normal Foaling

John J. Dascanio

96 Premature Separation of the Placenta

Patrick M. McCue

97 Dystocia Management

Patrick M. McCue and Sofie Sitters

98 Dystocia Correction

John J. Dascanio

99 Fetotomy

John J. Dascanio

100 Epidural

John J. Dascanio

101 Induction of Lactation to Create a Nurse Mare

Pouya Dini and Peter F. Daels

102 Screening the Pregnant Mare to Prevent Neonatal Isoerythrolysis

Patrick M. McCue

103 Evaluation of Colostrum Specific Gravity

Patrick M. McCue

104 Evaluation of Colostrum Quality: Brix Refractometry

Patrick M. McCue

105 Colostrum Banking

Patrick M. McCue

106 Obtaining Milk from the Mare

John J. Dascanio

107 Placental Evaluation

Patrick M. McCue

108 Removal of Retained Placenta

John J. Dascanio

109 Abdominocentesis in the Postpartum Mare

John J. Dascanio

110 Uterine Prolapse Treatment

John J. Dascanio

111 Buhner Needle Placement of a Perivulvar Suture

John J. Dascanio

112 Breeding Soundness Evaluation

Patrick M. McCue

113 Training of a Stallion to Use a Phantom

John J. Dascanio

114 Missouri Artificial Vagina

John J. Dascanio  and Lynda M.J. Miller

115 Colorado Model Artificial Vagina

Patrick M. McCue

116 Roanoke Artificial Vagina

John J. Dascanio

117 Hannover Artificial Vagina

Sofie Sitters

118 Teaser Stallions

Charles F. Scoggin

119 Standing Semen Collection

John J. Dascanio

120 Collection of Semen using an Open-Ended Artificial Vagina

Patrick M. McCue

121 Chemical Ejaculation

Patrick M. McCue

122 Preparation of a Jump Mare or a Mare for Natural Cover

John J. Dascanio

123 Washing the Penis

John J. Dascanio

124 Evaluation of Sexual Behavior in the Stallion

John J. Dascanio Sue M. McDonnell

125 Enhancement of Sexual Interest and Response in Stallions

Sue M. McDonnell

126 Management of Ejaculation Difficulty in Stallions

Sue M. McDonnell

127 Calibrated Spectrophotometer Evaluation of Sperm Concentration

John J. Dascanio

128 Hemocytometer Evaluation of Sperm Concentration

John J. Dascanio

129 NucleoCounter® Evaluation of Sperm Concentration and Viability

Patrick M. McCue

130 Visual Evaluation of Sperm Motility

Patrick M. McCue

131 Computer-Assisted Sperm Analysis

John J. Dascanio  and Lynda M. J. Miller

132 Eosin-Nigrosin Staining in the Evaluation of Sperm

Julie T. Cecere

133 Evaluation of Sperm Morphology

Leonardo Brito

134 Determination of Daily Sperm Output

Patrick M. McCue

135 Measurement of Testicular Size and Estimation of Daily Sperm Output

Patrick M. McCue

136 Wet Mount Evaluation of Sperm

John J. Dascanio

137 Diff-Quik  Evaluation of Round Cells and Sperm

John J. Dascanio and Lynda M. J. Miller

138 Electron Microscopy of Constituents of Seminal Ejaculates

D. N. Rao Veeramachaneni, BVSc, MScVet, PhD

139 Bacterial Culture in the Stallion

John J. Dascanio

140 Evaluation of pH and Osmolarity of Semen

Patrick M. McCue

141 Alkaline Phosphatase: A Marker for Ejaculation

Patrick M. McCue

142 Diagnosis and Management of Urospermia

John J. Dascanio

143 Hemospermia

Patrick M. McCue

144 Semen Extenders and Sperm Media

Patrick M. McCue

145  Preparation of Semen for Cooled Transport

Etta A. Bradecamp

146 Packing Semen for Cooled Transport

Etta A. Bradecamp

147 Longevity Testing of Sperm

John J. Dascanio

148 Calculation of g Force for Centrifuging Semen

John J. Dascanio

149 Centrifugation of Semen: Standard Technique

Etta A. Bradecamp

150 Centrifugation of Semen: Cushion Technique

Etta A. Bradecamp

151 Centrifugation of Semen: Selection of Motile Sperm Using a Single Layer Colloid Technique

Etta A. Bradecamp

152 Use of a Sperm Filter

Marco A. Alvarenga, Lorenzo G. T. M. Segabinazzi

153 Semen Freezing

Patrick M. McCue

154 Refreezing Stallion Semen

Patrick M. McCue

155 Thawing Frozen Semen

John J. Dascanio

156 Semen Processing for ICSI

Patrick M. McCue

157 Preparing a Vapor Shipper

Julie T. Cecere and Paul Loomis

158 Loading a Vapor Shipper

Julie T. Cecere and Paul Loomis

159 Maintaining a Long-Term Frozen Semen Storage Tank

John J. Dascanio

160 International Transport of Frozen Semen

Paul Loomis

161 Harvesting and Shipping Testes

John J. Dascanio

162 Epididymal Sperm Recovery

John J. Dascanio  and Lynda M.J. Miller

163 Endoscopic Examination of the Urethra

Patrick M. McCue

164 Endoscopic Guided Cannulation of the Seminal Vesicles

Patrick M. McCue

165 Palpation and Ultrasonography of the Testis, Epididymis, and Spermatic Cord

Sofie Sitters

166 Palpation and Ultrasonography of the Accessory Sex Glands

Sofie Sitters

167 Radiographic Examination of the Penis

Patrick M. McCue

168 Testicular Biopsy and Aspiration

John J. Dascanio

169 Assessment of Sperm Plasma Membrane Integrity and Viability: Propidium Iodide/SYBR-14

Amanda I. Glazar  and Patrick M. McCue

170 Sperm Chromatin Structure Assay

Charles Love

171 Hypo-Osmotic Swelling Test

Patrick M. McCue

172 Assessment of Sperm Acrosomal Status: FITC PNA

Amanda I. Glazar and Patrick M. McCue

173 Assessment of Sperm Mitochondrial Function: JC-1 and Rhodamine 123

Amanda I. Glazar and Patrick M. McCue

174 Equine Viral Arteritis Testing

G. Reed Holyoak and Udeni B. R. Balasuriya

175 Diagnostic Endocrinology: Baseline Hormone Levels

Patrick M. McCue

176 Diagnostic Endocrinology: GnRH Stimulation Tests

Patrick M. McCue

177 Diagnostic Endocrinology: hCG Stimulation Test

Patrick M. McCue

178 Diagnostic Endocrinology: Estrogen Conjugate Assay

Patrick M. McCue

179 Diagnostic Tests for Cryptorchidism

Patrick M. McCue

180 Probang Apparatus

Julie T. Cecere

181 Support Apparatus for Paraphimosis

John J. Dascanio

182 Equine Castration Techniques

Stacy Anderson

183 Stallion Acupuncture

Rhonda A Rathgeber

184 Birth Resuscitation

Patrick M. McCue

185 Foal Handling and Restraint

Undine Christmann

186 Madigan Foal Squeeze Technique

John Madigan

187 Evaluation of Passive Transfer

Patrick M. McCue

188 Colostral Administration via Naso-Gastric Intubation

John J. Dascanio

189 Sedation of the foal

Undine Christmann

190 Intravenous Catheter Placement in the Foal

Undine Christmann

191 Plasma Therapy in the Foal

Undine Christmann

192 Acupuncture in the Neonate

Peter Morresey

193 Routine Care

Patrick M. McCue

194 Foal Rejection

Patrick M. McCue

195 Fostering a Foal onto a Nurse Mare

Peter F Daels and Pouya Dini

196 Entropion

John J. Dascanio

197 Umbilical Ultrasound in the Foal

Undine Christmann

198 Management of Soft Tissue Laxity in Foals

Stacy Anderson

199 Management of Angular Limb Deformities

Stacy Anderson

200 Management of Congenital Flexural Limb Deformities

Stacy Anderson

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