Essential Developmental Biology A Practical Approach

by Stern, Claudio D.; Holland, Peter W. H.

• ISBN13: 9780199634224
• ISBN10: 019963422X
• Format: Paperback
• Copyright: 1993-09-30
• Publisher: IRL Press

Summary

Developmental biology is a newly-developing subject that is attracting much interest because of exciting findings being made using a combination of classical embryo manipulation with more modern techniques. This book brings together for the first time, and in comprehensive, easy-to-followprotocols, practical instructions for all of the main techniques, from traditional embryology to cellular and molecular methods. It includes complete reprints of all the stage tables in common use for the main laboratory species. It will become an essential addition to laboratory benches of thoseusing any technique as applied to embryos, cells, or tissues. It will also help teachers to design practical classes suitable for undergraduate and graduate courses.

Table of Contents

Basic techniques: obtaining and handling embryos

Drosophila embryos

Nematode embryos

Embryos and larvae of invertebrate deuterostomes

Zebrafish embryos

Xenopus

Avian embryos

Post-implantation mammalian embryos

Simple tips for photomicrography or embryos

Experimental Embryology

Fate maps and cell lineage analysis

Microinjection into Drosophila embryos

Cell transplantation in Xenopus

Transplantation in avian embryos

Chick limb buds

Nuclear transplantation

Laser ablation of cells

Studying cell movements in vivo by time-lapse video microscopy

Induction assays in Xenopus

Culture of avian neural crest cells

ES cells and gene targeting

Introduction of genes using retroviral vectors

Molecular techniques

Immunocytochemistry of embryonic material

RNase protection assays

cDNA library construction

Analysis of gene expression by RT-PCR

Cloning genes using PCR

In situ hybridisation

Appendices

Stage tables

Westerfield for zebrafish embryos

Nieukoop & Faber for Xenopus laevis

Eyal-Giladi & Kochav for early chick

Hamburger & Hamilton for later chick

Suppliers of specialist items

Index

Table of Contents provided by Publisher. All Rights Reserved.