Essential Zebrafish Methods: Cell and Developmental Biology

by Westerfield; Zon; Detrich, III

• ISBN13: 9780123745996
• ISBN10: 0123745993
• eBook ISBN(s): 9780080923437, 9780123745996
• Edition: 1st
• Format: Paperback
• Copyright: 2009-09-10
• Publisher: Elsevier Science
• More Book Details
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Summary

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues which make it the model organism for study of vertebrate development. This book will provide overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques. The methods and techniques in this volume were chosen by the editors of Methods in Cell Biology, whose goal was to provide fail-safe methods, tips, and "tricks of the trade" to experienced researchers and more junior members in the lab. * Provides busy researchers a quick reference for time-tested methods and protocols that really work. * Includes "quick tips and tricks" for each method. * Provides pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error.

Table of Contents

Overview of the Zebrafish System

Detrich III Cell Cycles and Development in the Embryonic Zebrafish

Primary Fibroblast Cell Culture

Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization)

Analysis of Protein and Gene Expression Trevor Jowett Analysis of Zebrafish Development Using Explant Culture Assays Hazel Sive Confocal Microscopic Analysis of Morphogenetic Movements

Cytoskeletal Dynamics of the Zebrafish Embryo Lilianna Solinica-Krezel Haploid Screens and Gamma-Ray Mutagenesis Charline Walker Positional Cloning of Mutated Zebrafish Genes Will Talbot The Zebrafish Genome

Analyzing Axon Guidance in the Zebrafish Retinotectal System

Analysis of the Cell Cycle in Zebrafish Embryos

Cellular Dissection of Zebrafish Hematopoiesis

Culture of Embryonic Stem Cell Lines from Zebrafish

Neurogenesis Laure Bally-Cuif Time-Lapse Microscopy of Brain Development

Development of the Peripheral Sympathetic Nervous System in Zebrafish

Approaches to Study Neurogenesis in the Zebrafish

Instrumentation for Measuring Oculomotor Performance and Plasticity in Larval Organisms

Development of Cartilage and Bone

Morphogenesis of the Jaw: Development Beyond the Embryo

Cardiac Development Didier Y. R. Stainier Zebrafish Kidney Development

Retroviral-Mediated Insertional Mutogenesis in Zebrafish

Genetic Screens for Maternal-Effect Mutations

Behavior Screening Assays in Zebrafish

A High-Throughput Method for Identifying N-Ethyl-N-Nitrosourea (ENU)-Induced Point Mutations in Zebrafish

Transgenesis and Gene Trap Methods in Zebrafish by Using the Tol2 Transposable Element

Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC)Probe Panel Resource for Genomic Analysis in Zebrafish

The Zon Laboratory Guide to Positional Cloning in Zebrafish

Sleeping Beauty Transposon for Efficient Gene Delivery

Transgene Manipulation in Zebrafish by Using Recombinases

Highly Efficient Zebrafish Transgenesis Mediated by the Meganuclease I-SceI Joachim Wittbrodt Cloning Zebrafish by Nuclear Transfer Shuo Lin Spatial and Temporal Expression of the Zebrafish Genome by Large-Scale

In Situ Hybridization Screening Christine Thisse Genetic Backgrounds, Standard Lines, and Husbandry of Zebrafish Bill Trevarrow Common Diseases of Laboratory Zebrafish

Zebrafish Sperm Cryopreservation

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