Essentials of Chemical Biology Structure and Dynamics of Biological Macromolecules

by Miller, Andrew D.; Tanner, Julian

• ISBN13: 9780470845318
• ISBN10: 0470845317
• eBook ISBN(s): 9780470987155
• Additional ISBN(s): 9781119437970
• Edition: 1st
• Format: Paperback
• Copyright: 2008-08-11
• Publisher: WILEY
• More Book Details

Summary

"This excellent work fills the need for an upper-level graduate course resource that examines the latest biochemical, biophysical, and molecular biological methods for analyzing the structures and physical properties of biomolecules... This reviewer showed [the book] to several of his senior graduate students, and they unanimously gave the book rave reviews. Summing Up: Highly recommended..." CHOICEChemical biology is a rapidly developing branch of chemistry, which sets out to understand the way biology works at the molecular level. Fundamental to chemical biology is a detailed understanding of the syntheses, structures and behaviours of biological macromolecules and macromolecular lipid assemblies that together represent the primary constituents of all cells and all organisms. The subject area of chemical biology bridges many different disciplines and is fast becoming an integral part of academic and commercial research.This textbook is designed specifically as a key teaching resource for chemical biology that is intended to build on foundations lain down by introductory physical and organic chemistry courses. This book is an invaluable text for advanced undergraduates taking biological, bioorganic, organic and structural chemistry courses. It is also of interest to biochemists and molecular biologists, as well as professionals within the medical and pharmaceutical industry.Key Features: A comprehensive introduction to this dynamic area of chemistry, which will equip chemists for the task of understanding and studying the underlying principles behind the functioning of biological macro molecules, macromolecular lipid assemblies and cells. Covers many basic concepts and ideas associated with the study of the interface between chemistry and biology. Includes pedagogical features such as: key examples, glossary of equations, further reading and links to websites. Clearly written and richly illustrated in full colour.

Author Biography

Professor Andrew David Miller, Imperial College Genetic Therapies Centre, Imperial College of  Science, Technology and Medicine, London, UK.

Dr Julian A. Tanner, Department of Biochemistry, University of Hong Kong.

Table of Contents

Preface

Glossary of physical terms

The structures of biological macromolecules and lipid assemblies

General introduction

Protein structure

Carbohydrate structure

Nucleic acid structure

Macromolecular lipid assemblies

Structural forces in biological macromolecules

Chemical and biological synthesis

Introduction to synthesis in chemical biology

Chemical synthesis of peptides and proteins

Chemical synthesis of nucleic acids

Chemical synthesis of oligosaccharides

Chemical synthesis of lipids

Biological synthesis of biological macromolecules

Directed biological synthesis of proteins

Biological syntheses of nucleic acids, oligosaccharides and lipids

Molecular biology as a toolset for chemical biology

Key concepts in molecular biology

Tools and techniques in molecular biology

Cloning and identification of genes in DNA

Integrating cloning and expression

Site-directed mutagenesis

Electronic and vibrational spectroscopy

Electronic and vibrational spectroscopy in chemical biology

UV-visible spectroscopy

Circular dichroism spectroscopy

Vibrational spectroscopy

Fluorescence spectroscopy

Probing metal centres by absorption spectroscopy

Magnetic resonance

Magnetic resonance in chemical biology

Key principles of NMR

Two-dimensional NMR

Multi-dimensional NMR

Biological macromolecule structural information

EPR spectroscopy; key principles

Diffraction and microscopy

Diffraction and microscopy in chemical biology

Key principles of X-ray diffraction

Structural information from X-ray diffraction

Neutron diffraction

Key principles of electron microscopy

Key principles of scanning probe microscopy

Molecular recognition and binding

Molecular recognition and binding in chemical biology

Theoretical models of binding

Analysing molecular recognition and binding

Biological molecular recognition studies

Kinetics and catalysis

Catalysis in chemical biology

Steady state kinetic schemes

Pre-steady-state kinetics

Theories of biocatalysis

Electron transfer

Mass spectrometry and proteomics

Mass spectrometry in chemical biology

Key principles in mass spectrometry

Structural analysis of biological macromolecules and lipids by mass spectrometry

The challenge of proteomics

Genomics - assigning function to genes and proteins

Molecular selection and evolution

Chemical biology and the origins of life

Molecular breeding; natural selection acting on self-organisation

Directed evolution of protein function

Directed evolution of nucleic acids

Catalytic antibodies

References

Index

Table of Contents provided by Publisher. All Rights Reserved.