Experimental Glycoscience

by Taniguchi, Naoyuki; Suzuki, Akemi; Ito, Yukishige; Narimatsu, Hisashi; Kawasaki, Toshisuke

• ISBN13: 9784431779230
• ISBN10: 443177923X
• Edition: 1st
• Format: Paperback
• Copyright: 2008-08-01
• Publisher: Springer Nature

Summary

The aim of this book is to provide experimental protocols covering many aspects of glycobiology, glycotechnology, and chemistry: biochemistry, molecular and cellular biology, genetics, physiology, and medicine. The protocols are all self-contained descriptions of the equipment and reagents needed, followed by details of the experimental procedure. In the post-genomic era, glycobiology is coming of age because more than half of proteins are glycosylated and the importance of sugar chains in various fields of life science research cannot be disregarded. Many scientists had not entered this area because glycobiology and glycoscience used to be considered difficult fields. This book, therefore, is presented much like a cookbook which can help scientists in fields other than glycobiology and glycoscience carry out research more easily.

Table of Contents

Structural Analysis of Sugar Chains
Release of Sugar Chains and Labeling
Chemical Liberation of N-linked Oligosaccharides from Glycoproteins	p. 5
Release of N-glycans by Enzymatic Methods	p. 7
Release of O-glycans by Chemical Methods	p. 12
Release of O-glycans by Enzymatic Methods	p. 14
Ceramidase and Related Enzymes	p. 18
Labeling of Oligosaccharides	p. 22
Chemical Labeling of Sialyloligo/polymer	p. 24
Separation of Oligosaccharides by 2D HPLC	p. 28
Analysis of Oligosaccharides by Capillary Electrophoresis	p. 30
Sequence Analysis
Energy-Resolved Mass Spectrometry (ERMS) of Oligosaccharide	p. 37
LC/MS of N-Linked Oligosaccharides	p. 39
Convenient Structural Characterization of Intact Glycosphingolipids by MALDI-TOF Mass Spectrometry with Increased Laser Power and Cooling Gas Flow	p. 42
Structural Analyses of Glycoconjugates by NMR	p. 45
Sugar Chain Analysis by Enzymatic Digestion and 2D Mapping by HPLC	p. 51
Analysis of Binding Sites of Sugar Chains by Methylation Analysis	p. 54
Glycan Profiling	p. 56
Monoclonal Antibody as a Clue to Structural Analysis of Bioactive Functional Glycoconjugates	p. 60
Microsequencing of Functional Chondroitin Sulfate Oligosaccharides	p. 64
Structural Characterization of PA-oligosaccharide Isomers Derived from Glycosphingolipids by MALDI-TOF Mass Spectrometry	p. 70
Structural Analysis of Phospho-Glycosphingolipids in Lower Animals	p. 74
Structural Analysis of Polysialic Acid	p. 77
Milk Oligosaccharides: Structural Characterization and Future Aspects	p. 82
Sugar Chain Analysis by Mass Spectrometry
An XML Description of Carbohydrate Structures	p. 89
Construction of a Diagnostic Library for Glycans Using Multistage Tandem Mass Spectrometry (MS")	p. 92
A Method for Large-Scale Analysis for N-linked Glycoproteins by the Glycosylation Site-Specific Stable Isotope-Labeling and LC/MS Shotgun Technology	p. 94
Mass Spectrometry of Glycopeptides	p. 98
Development of MS Method for the Analysis of Sugar Peptide	p. 100
Calculations for Saccharides by the Use of MS Data	p. 105
Analysis of the Three-dimensional Structure of Sugar Chains
Molecular Modeling of Oligosugar Structures	p. 109
Prediction of Sugar-Binding Sites on Proteins	p. 111
Analysis of Sugar-Protein Interactions
Frontal Affinity Chromatography: An Effective Analytical Tool for Protein-Sugar Interaction	p. 117
Analyses of Sugar-Protein Interactions by NMR	p. 121
Surface Plasmon Resonance and Sugar Chip Analysis for Sugar Chain- Protein Interactions	p. 124
Analysis of Interactions Between Carbohydrates and Proteins Using Capillary Affinity Electrophoresis	p. 127
Equilibrium Dialysis	p. 132
Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS)	p. 134
Development of Neoglycoconjugate Probes and Detection of Lectins	p. 138
Chemical Synthesis of Sugar Chains
Chemical Synthesis of Sugar Chains
Synthesis of Glycolipid and Its Application	p. 147
Chemo-Enzymatic Synthesis of Glycoconjugates	p. 151
Construction of O-Linked Glycopeptide Library Using Human Glycosyltransferases	p. 155
Alteration of N-Linked Oligosaccharides	p. 158
Stationary Solid-Phase Reaction (SSPR) for Oligosaccharide Synthesis	p. 164
Sugar Chain Synthesis by the Use of Cell Functions	p. 166
Glyco-Chemistry Cycle System Based on Glycosidases	p. 169
Endoglycosidases (Glycosaminoglycans)	p. 173
Oligosaccharide Synthesis Based on Combinatorial Chemistry and Labo Automation	p. 177
Endoglycosidases (Glycoproteins)	p. 182
Glycosaminoglycans	p. 186
Recent Advances in the Production of Mammalian-Type Sugar Chains in Yeast	p. 191
Solid-Phase Synthesis of Glycopeptides	p. 195
Efficient Synthesis of Oligosaccharides and Synthesis of Pathogen-Associated Molecular Patterns for Their Biofunctional Studies	p. 200
Sugar Polymers (Dendrimers and Pendant-Type Linear Polymers)	p. 206
Rapid Synthesis of Oligosaccharides: Resin Capture-Release Strategy	p. 210
Glossary	p. 217
Index	p. 249
Table of Contents provided by Blackwell. All Rights Reserved.