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9780521478922

Experiments in Plant Tissue Culture

by ;
  • ISBN13:

    9780521478922

  • ISBN10:

    0521478928

  • Edition: 3rd
  • Format: Paperback
  • Copyright: 1995-02-01
  • Publisher: Cambridge Univ Pr

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Summary

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.

Table of Contents

Part I. Background Information: 1. Culture of plant cells, tissues, and organs
2. Laboratory facilities
3. Aseptic techniques
4. Media composition and preparation
Part II. Experimental: Callus and Callus-Derived Systems: 5. Initiation and maintenance of callus
6. Organogenesis
7. Cell suspensions
8. Somatic embryogenesis
Part III. Experimental: Culture of Organs and Organized Systems: 9. Isolated roots
10. Micropropagation by bud proliferation
11. Anther and pollen cultures
Part IV. Experimental: Isolated Cells: 12. Transdifferentiation of parenchyma cells to tracheary elements
13. Isolation and culture of protoplasts
14. Protoplast fusion and somatic hybridization
Part V. Supplementary Topics: 15. Cryopreservation of germplasm
16. Production of secondary metabolites
17. Quantitation of procedures
18. Formulations of tissue culture media
19. Commercial sources of supplies
Author index
Subject index.

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