Fish Technology: Lab Manual

by Rautenstrauss, Bernd W.; Liehr, Thomas

• ISBN13: 9783540672760
• ISBN10: 3540672761
• Format: Paperback
• Copyright: 2002-04-01
• Publisher: Springer Verlag

Summary

Fluorescence in situ hybridization (FISH) is a powerful method to directly visualise the localisation of genomic alterations in the nucleus. The technique has been adapted to a wide range of applications in both medicine, especially diagnostic cytogenetics, and biology. Topics described in this manual include: FISH on native human tissues, such as blood, bone marrow, epithelial cells, hair root cells, amniotic fluid cells, human sperm cells; FISH on archival human tissues, e.g. formalin-fixed and paraffin-embedded tissue sections, cryofixed tissue; simultaneous detection of apoptosis and expression of apoptosis-related genes; comparative genomic hybridization; special FISH techniques (Fiber-, PNA-, CO-, NU- and DBD-FISH); FISH on insect cells and yeast; multi-color FISH applications (spectral karyotyping analysis, reverse FISH and FICTION); FISH-related techniques, such as PRINS and microarrays.

Table of Contents

The Standard FISH Procedure - an Introduction
Fish Techniques, Fish Probes and Their Applications in Medicine and Biology - An Overview	3	(48)
Eigil Kjeldsen
Steen Kølvraa
Microscopy and Imaging Systems	51	(22)
Andreas Plesch
FISH on Native, Human Tissues
Fish on Chromosome Preparations of Peripheral Blood	73	(9)
Thomas Liehr
Uwe Claussen
Fish on Uncultured Blood or Bone Marrow	82	(8)
Thomas Liehr
Fish on Blood or Bone Marrow Smears	90	(7)
Thomas Liehr
Epithelial Cells from Buccal Smears and Urine	97	(1)
Anita Rauch
Fish on Hair Root Cells	97	(18)
Thomas Liehr
Amniotic Fluid Cells - Uncultured	115	(12)
Renate Ulmer
Human Sperm Cells	127	(14)
Anita Rauch
FISH on Archival, Human. Tissues
Archival Cytogenetic Slides	141	(7)
Erich Gebhart
Formalin-Fixed and Paraffin-Embedded Tissue Sections	148	(14)
Christine Sommerlad
Yasmin Mehraein
Marina Giersberg
Kersten Marben
Harald Rieder
Helga Rehder
Nucleus Extraction from Formalin Fixed/paraffin Embedded Tissue	162	(9)
Thomas Liehr
Combined in Situ Nick Translation and mRNA Fish for Simultaneous Detection of Apoptosis and Expression of Apoptosis-Related Genes in Sections from Paraffin-Embedded Tissue	171	(5)
Norbert Heckmann
Nucleus Extraction from Cryofixed Tissue	176	(9)
Bernd Rautenstrauss
Thomas Liehr
Preparation of Samples for CGH by DOP-PCR from Cryofixed or Paraffin-Embedded, Microdissected Cells	185	(12)
Volker Jung
Georg A. Weber
Comparative Genomic Hybridization	197	(24)
Burt G. Feuerstein
Yuichi Hirose
Malgorzata Pellarin
Gregg Magrane
Special FISH Techniques (Fiber-FISH, PNA-FISH, CO-FISH, NU-FISH)
High Resolution Fish of Stretched Chromosome Fibers	221	(5)
Bernd Rautenstrauss
Christina Fuchs
Quantitative DNA Fiber Mapping	226	(28)
Heinz-Ulrich G. Weier
PNA-Fish	254	(8)
William M. Strauss
Strand-Specific Fluorescence in Situ Hybridization: CO-FISH and COD-FISH	262	(10)
Julianne Meyne
Susan M. Bailey
Edwin H. Goodwin
The Combined Use of Nuclease in Situ Digestion and Fish (NU-FISH)	272	(10)
Jaime Gosalvez
Joaquina De La Torre
Carmen Lopez-Fernandez
Jose Luis Fernandez
DNA Breakage Detection-Fish (DBD-FISH)	282	(9)
Jose Luis Fernandez
Vicente Goyanes
Jaime Gosalvez
Detection of Radiosensitivity by Means of Three-Color Fish	291	(10)
Susann Neubauer
Fluorescence in Situ Hybridisation of a Complete Set of 41 Telomere Specific Probes in a Single Simultaneous Hybridisation Experiment Using the Chromoprobe Multiprobe System-T	301	(8)
Martin Lawrie
Maria Ocana Gil
Don Cardy
FISH in Zoology and Virology
Zoo-Fish	309	(14)
Harry Scherthan
Fish on Insect Cells Transfected with Heterologous DNA	323	(6)
Thomas Liehr
Yeast Fish: Delineation of Chromosomal Targets in Vegetative and Meiotic Yeast Cells	329	(17)
Harry Scherthan
Edgar Trelles-Sticken
In Situ Detection of Viral Nucleic Acids by Chemiluminescence	346	(17)
Monica Musiani
Patrizia Pasini
Aldo Roda
Marialuisa Zerbini
Multicolor FISH Applications
Spectral Imaging Analysis of Metaphase and Interphase Cells	363	(25)
Jingly Fung
Heinz-Ulli G. Weier
Roger A. Pedersen
Horst F. Zitzelsberger
Microdissection of Chromosomes and Reverse Fish	388	(20)
Ilse Chudoba
Gabriele Senger
Multicolor Fish Techniques for the Detection of Inter- and Intrachromosomal Rearrangements	408	(17)
Horst Zitzelsberger
Benjamin O'Brien
Heinz-Ulrich Weier
Centromere-Specific Multicolor-Fish (cenM-FISH)	425	(7)
Angela Nietzel
Anita Heller
Heike Starke
Uwe Clausen
Thomas Liehr
Simultaneous Multicolor-FISH and Immunocyto-chemical Analysis of Fresh Tumor Material	432	(10)
Heike Steilen-Gimbel
Steffi Urbschat
Simultaneous Fluorescence Immunophenotyping and Fish (Fiction)	442	(21)
Brigitte Schlegelberger
Stefan Gesk
Yanming Zhang
Reiner Siebert
Not FISH but Almost
PRINS: Hybridization and Primed in Situ Labeling in One Step	463	(10)
Jorn Koch
Microarrays and DNA-Chips in the Analysis of Genetic Abberrations	473	(12)
Uwe R. Muller
Y. Paul Bao
Guangshan Li
Xinying Xie
Nalini Raghavachari
Appendix	485	(2)
Subject index	487