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9780195152340

Flow Cytometry for Biotechnology

by
  • ISBN13:

    9780195152340

  • ISBN10:

    0195152344

  • Format: Paperback
  • Copyright: 2005-09-02
  • Publisher: Oxford University Press

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Summary

Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in thelaser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry aversatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant andreproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

Author Biography


Larry Sklar is Regents Professor of Pathology at the University of New Mexico. He is also Director of Basic Research at the University of New Mexico Cancer Research and Treatment Center.

Table of Contents

Contributors ix
The Future of Flow Cytometry in Biotechnology
3(12)
Larry A. Sklar
Part I Tools for Flow Cytometry
Fluorescent Probes
15(25)
Howard M. Shapiro
Automation and High-Throughput Flow Cytometry
40(17)
Bruce S. Edwards
Larry A. Sklar
Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies
57(11)
David Basiji
Elastomeric Microfabricated Fluorescence-Activated Cell Sorters
68(37)
Anne Y. Fu
Yohie Yokobayashi
Frances H. Arnold
Stephen R. Quake
Flow Cytometry, Beads, and Microchannels
105(18)
Tione Buranda
Larry A. Sklar
Gabriel P. Lopez
DNA Fragment Sizing by High-Sensitivity Flow Cytometry: Applications in Bacterial Identification
123(16)
Babetta L. Marrone
Robert C. Habbersett
James H. Jett
Richard A. Keller
Xiaomei Yan
Thomas M. Yoshida
A Guide to Informatics and Flow Cytometry
139(14)
Adam Treister
Molecular Assemblies, Probes, and Proteomics in Flow Cytometry
153(28)
Steven W. Graves
John P. Nolan
Larry A. Sklar
Part II Biological Applications
Multiparameter Analysis: Application to Vaccine Analysis
181(12)
Mario Roederer
Stephen C. De Rosa
FACS-Based High-Throughput Functional Screening of Genetic Libraries for Drug Target Discovery
193(19)
Erlina Pali
Mark Powell
Esteban Masuda
Yasumichi Hitoshi
Sacha Holland
Mark K. Bennett
Susan M. Molineaux
Donald G. Payan
James B. Lorens
Applications of Flow Cytometry in Protein Engineering
212(18)
George Georgiou
Barrett R. Harvey
Karl E. Griswold
Brent L. Iverson
Applications of Flow Cytometry in Animal Reproduction
230(29)
Duane L. Garner
George E. Seidel, Jr.
Genetic Analysis Using Microsphere Arrays
259(16)
John P. Nolan
Alina Desphande
Feng Zhou
Uses of Flow Cytometry in Preclinical Safety Pharmacology and Toxicology
275(16)
Scott W. Burchiel
James L. Weaver
Flow Cytometry and Cell Sorting in Plant Biotechnology
291(32)
David W. Galbraith
Jan Bartos
Jaroslav Dolezel
Analysis of GTP-Binding Protein-Coupled Receptor Assemblies by Flow Cytometry
323(24)
Peter Simons
Charlotte M. Vines
T. Alexander Key
Ross M. Potter
Mei Shi
Larry A. Sklar
Eric R. Prossnitz
Applications of Flow Cytometry to Cell Adhesion Biology: From Aggregates to Drug Discovery
347(28)
Richard S. Larson
Alexandre Chigaev
Bruce S. Edwards
Sergio A. Ramirez
Stuart S. Winter
Gordon Zwartz
Larry A. Sklar
Index 375

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