Flow Cytometry For Biotechnology

by Sklar, Larry A.

• ISBN13: 9780195183146
• ISBN10: 0195183142
• Format: Hardcover
• Copyright: 2005-09-02
• Publisher: Oxford University Press

Summary

Flow cytometry is a sensitive and quantitative platform for the measurement of particle fluorescence. In flow cytometry, the particles in a sample flow in single file through a focused laser beam at rates of hundreds to thousands of particles per second. During the time each particle is in thelaser beam, on the order of ten microseconds, one or more fluorescent dyes associated with that particle are excited. The fluorescence emitted from each particle is collected through a microscope objective, spectrally filtered, and detected with photomultiplier tubes. Flow cytometry is uniquely capable of the precise and quantitative molecular analysis of genomic sequence information, interactions between purified biomolecules and cellular function. Combined with automated sample handling for increased sample throughput, these features make flow cytometry aversatile platform with applications at many stages of drug discovery. Traditionally, the particles studied are cells, especially blood cells; flow cytometry is used extensively in immunology. This volume shows how flow cytometry is integrated into modern biotechnology, dealing with issues of throughput, content, sensitivity, and high throughput informatics with applications in genomics, proteomics and protein-protein interactions, drug discovery, vaccine development, plant andreproductive biology, pharmacology and toxicology, cell-cell interactions and protein engineering.

Table of Contents

Contributors	ix
1. The Future of Flow Cytometry in Biotechnology	3	(12)
Larry A. Sklar
Part I Tools for Flow Cytometry
2. Fluorescent Probes	15	(25)
Howard M. Shapiro
3. Automation and High-Throughput Flow Cytometry	40	(17)
Bruce S. Edwards and Larry A. Sklar
4. Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies	57	(11)
David Basiji
5. Elastomeric Microfabricated Fluorescence-Activated Cell Sorters	68	(37)
Anne Y. Fu, Yohie Yokobayashi, Frances H. Arnold, and Stephen R. Quake
6. Flow Cytometry, Beads, and Microchannels	105	(18)
Tione Buranda, Larry A. Sklar, and Gabriel P. Lopez
7. DNA Fragment Sizing by High-Sensitivity Flow Cytometry: Applications in Bacterial Identification	123	(16)
Babetta L. Marrone, Robert C. Habbersett, James H. Jett, Richard A. Keller, Xiaomei Yan, and Thomas M. Yoshida
8. A Guide to Informatics and Flow Cytometry	139	(14)
Adam Treister
9. Molecular Assemblies, Probes, and Proteomics in Flow Cytometry	153
Steven W. Graves, John P. Nolan, and Larry A. Sklar
Part II Biological Applications
10. Multiparameter Analysis: Application to Vaccine Analysis	181	(12)
Mario Roederer and Stephen C. De Rosa
11. FACS-Based High-Throughput Functional Screening of Genetic Libraries for Drug Target Discovery	193	(19)
Erlina Pali, Mark Powell, Esteban Masuda, Yasumichi Hitoshi, Sacha Holland, Mark K. Bennett, Susan M. Molineaux, Donald G. Payan, and James B. Lorens
12. Applications of Flow Cytometry in Protein Engineering	212	(18)
George Georgiou, Barrett R. Harvey, Karl E. Griswold, and Brent L. Iverson
13. Applications of Flow Cytometry in Animal Reproduction	230	(29)
Duane L. Garner and George E. Seidel, Jr.
14. Genetic Analysis Using Microsphere Arrays	259	(16)
John P. Nolan, Alina Desphande, and Feng Zhou
15. Uses of Flow Cytometry in Preclinical Safety Pharmacology and Toxicology	275	(16)
Scott W. Burchiel and James L.Weaver
16. Flow Cytometry and Cell Sorting in Plant Biotechnology	291	(32)
David W. Galbraith, Jan Bartoš, and Jaroslav Doležel
17. Analysis of GTP-Binding Protein-Coupled Receptor Assemblies by Flow Cytometry	323	(24)
Peter Simons, Charlotte M. Vines, T. Alexander Key, Ross M. Potter, Mei Shi, Larry A. Sklar, and Eric R. Prossnitz
18. Applications of Flow Cytometry to Cell Adhesion Biology: From Aggregates to Drug Discovery	347	(28)
Richard S. Larson, Alexandre Chigaev, Bruce S. Edwards, Sergio A. Ramirez, Stuart S. Winter, Gordon Zwartz, and Larry A. Sklar
Index	375