Flow Cytometry Protocols

by Hawley, Teresa S.; Hawley, Robert G.

• ISBN13: 9781617379499
• ISBN10: 1617379492
• Edition: 3rd
• Format: Hardcover
• Copyright: 2010-12-30
• Publisher: Humana Pr Inc

Summary

With rapid improvements in instrumentation, lasers, fluorophores, and data analysis software, flow cytometry is riding the crest of unprecedented, innovative advances. This thoroughly revised and up-to-date third edition of Flow Cytometry Protocols highlights the expanding contribution of flow cytometry to basic biological research and diagnostic medicine. Written by leading experts in the field, the book presents cutting-edge topics such as polychromatic, quantitative, and high throughput flow cytometry, novel multiparametric data analysis which breaks the dimensionality barrier, standard practice and safety measures for aerosol-generating cell sorting, conventional and imaging flow cytometry as well as minimalist imaging cytometry. As a volume in the highly successful Methods in Molecular Biology'„¢ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.Authoritative and comprehensive, Flow Cytometry Protocols, Third Edition presents established as well as new flow cytometric methodologies in order to introduce beginning users to basic applications while opening new avenues of innovation for seasoned users.

Table of Contents

Preface	p. v
Contributors	p. ix
Flow Cytometry: An Introduction	p. 1
Breaking the Dimensionality Barrier	p. 31
Quantitative Fluorescence Measurements with Multicolor Flow Cytometry	p. 53
Quantum Dots for Quantitative Flow Cytometry	p. 67
Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry	p. 85
Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization	p. 97
Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry	p. 119
Multiparameter Intracellular Cytokine Staining	p. 165
Phospho Flow Cytometry Methods for the Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples	p. 179
Multiparametric Analysis of Apoptosis by Flow Cytometry	p. 203
Multiparameter Cell Cycle Analysis	p. 229
Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue	p. 251
Flow Cytometry-Based Identification of Immature Myeloerythroid Development	p. 275
Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia	p. 295
Flow Cytometry Assays in Primary Immunodeficiency Diseases	p. 317
Flow Cytometric Analysis of Microparticles	p. 337
Noncytotoxic DsRed Derivatives for Whole-Cell Labeling	p. 355
Flow Cytometric FRET Analysis of Protein Interaction	p. 371
Fluorescent Protein-Assisted Purification for Gene Expression Profiling	p. 393
Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type	p. 407
Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics	p. 431
Standard Practice for Cell Sorting in a BSL-3 Facility	p. 449
The Cytometric Future: It Ain't Necessarily Flow!	p. 471
Index	p. 483
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