Forensic and Clinical Applications of Solid Phase Extraction

by Telepchak, Michael J.; Chaney, Robert; August, Thomas F.; Chaney, Glynn; August, Thomas F.

• ISBN13: 9780896036482
• ISBN10: 0896036480
• Format: Hardcover
• Copyright: 2004-05-01
• Publisher: Humana Pr Inc

Summary

This complete laboratory reference manual explains the principles behind solid phase extraction (SPE) and provides readily reproducible protocols for solving extraction problems in forensic and clinical chemistry. Numerous actual chromatograms, based on original research and diverse applications, demonstrate the technique and the results that can be achieved. Extensive appendices allow fast access to frequently needed information on reagents, the preparation of solutions and buffers, milliequivalent and millimode calculations, buffers and pKa for SPE, and a complete RapidTrace® technical manual. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and on avoiding known pitfalls.

Table of Contents

Preface	v
Dedication	vii
Acknowledgments	xi
Introduction to Solid Phase Extraction	1	(40)
Silica-Based Solid Phase Extraction	41	(14)
The Chemistry of Bonded Silicas	55	(8)
Bonded Phase Sorbent Synthesis	63	(28)
Strategies for the Use of Solid Phase Extraction	91	(18)
Optimizing Solid Phase Extraction Methods	109	(20)
Separation of Analytes from Their Matrices	129	(18)
Solid Phase Extraction Troubleshooting	147	(22)
Drug Methods for the Toxicology Lab	169	(76)
Selected Solid Phase Extraction Applications in Drug Analysis	245	(36)
General Methods for Drug Isolation	281	(16)
Chemical Derivatization	297	(14)
Appendix A: Glossary	311	(4)
Appendix B: Reagents	315	(2)
Appendix C: Preparation of Solutions and Buffers	317	(4)
Appendix D: Milliequivalent and Millimole Calculations	321	(2)
Appendix E: Selected Solvent Polarity and Property Scale	323	(2)
Appendix F: Buffers for Solid Phase Extraction	325	(2)
Appendix G: pKa Reference for Solid Phase Extraction	327	(8)
Appendix H: RapidTrace® Operating Guide	335	(8)
Appendix I: Further Reading	343	(8)
Index	351