Free Radicals A Practical Approach

by Punchard, Neville; Kelly, Frank

• ISBN13: 9780199635597
• ISBN10: 0199635595
• Format: Paperback
• Copyright: 1996-10-10
• Publisher: IRL Press

Summary

With the recognition that oxygen and nitrogen radicals are involved in normal cell metabolism, free radical research has begun to feature in most disciplines in the life sciences. Increasingly, their implication in a number of human disease processes is being accepted, altough conclusive evidence is lacking in many instances, partly due to the difficulties in detecting and measuring free radicals that have lifetimes measured in microseconds. This book outlines the latest techniques for quantifying radicals and their effects, and features detailed protocols, hints, and tips for success, troubleshooting comments, sample data, and key literature citations.

Table of Contents

Introduction

Direct and indirect measurements of free radicals by physico-chemical methods

In vitro and in vivo detection of free radical metabolites with electron spin resonance

The application of high-field nuclear magnetic resonance spectroscopy to the detiction of products arising from the reactions of free radicals in chemical model systems, biosystems, and foodstuffs

Pulse radiolysis

Direct and indirect measurement of free radicals by other methods

Intrinsic (low-level) chemiluminescence

Visual assessment of oxidative stress by multifunctional digital microfluorography

Salicylic acid and phenylalanine as probes to detect hydroxyl radicals

Measurement of free radical products

Lipids (i): peroxides and other products

Lipids (ii): quantitative analysis of 4-hydroxyl 2-nonenal

Lipids (iii): F2-isoprostanes: prostaglandin-like products of lipik peroxidation

Proteins (i): determination of carbonyl groups in oxidized proteins

Proteins (ii): protein hydroperoxides, protein hydroxides, and protein-bound DOPA

Carbohydrates: investigating the effects of oxygen free radicals and carbohydrates in biological systems

Nucleic acids: measurements of products of free radical attack on nucleic acids

Measurement of antioxidants

Glutahione

Glutathione peroxidase: activity and steady-state level of mRNA

Superoxide dismutase

Application of deuterium labelling and gas chromatographt-mass spectrometry to the measurement of vitamin E bioavailability in tissue and body fluids

Selective and sensitive measurement of vitamin C, ubiquinol-10, and other low molecular weight antioxidants

Measurement of antioxidant gene expression

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