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Summary

Rapid advances in a collection of techniques referred to as gene technology, genetic engineering, recombinant DNA technology and gene cloning have pushed molecular biology to the forefront of the biological sciences. This new edition of a concise, well-written textbook introduces key techniques and concepts involved in cloning genes and in studying their expression and variation. The book opens with a brief review of the basic concepts of molecular biology, before moving on to describe the key molecular methods and how they fit together. This ranges from the cloning and study of individual genes to the sequencing of whole genomes, and the analysis of genome-wide information. Finally, the book moves on to consider some of the applications of these techniques, in biotechnology, medicine and agriculture, as well as in research that is causing the current explosion of knowledge across the biological sciences. From Genes to Genomes: Concepts and Applications of DNA Technology, Second Edition includes full two-colour design throughout and an accompanying website. Specific changes for the new edition include: Strengthening of gene to genome theme Updating and reinforcing of material on proteomics, gene therapy and stem cells More eukaryotic/mammalian examples and less focus on bacteria This textbook is must-have for all undergraduates studying intermediate molecular genetics within the biological and biomedical sciences. It is also of interest for researchers and all those needing to update their knowledge of this rapidly moving field. From the reviews of the first edition: "This monograph is excellently organized.... an outstanding addition to the library of an undergraduate or graduate student and a useful aid for the libraries of mentors of undergraduate studies." -BIOCHEMISTRY AND MOLECULAR EDUCATION "...this lucid book provides very nice descriptions of many of the modern methods in molecular biology.." -ASM NEWS

Preface
Introduction
Basic molecular biology
Nucleic acid structure
What is a gene?
Information flow: gene expression
Gene structure and organization
How to clone a gene
What is cloning?
Overview of the procedures
Gene libraries
Hybridization
Polymerase chain reaction
Extraction and purification of nucleic acids
Detection and quantitation of nucleic acids
Gel electrophoresis
Cutting and joining DNA
Restriction endonucleases
Ligation
Modification of restriction fragment ends
Other ways of joining DNA molecules
Vectors
Plasmid vectors
Vectors based on the lambda bacteriophage
Cosmids
M13 vectors
Expression vectors
Vectors for cloning and expression in eukaryotic cells
Supervectors: YACs and BACs
Summary
Genomic and cDNA libraries
Genomic libraries
Growing and storing libraries
cDNA libraries
Random, arrayed and ordered libraries
Finding the right clone
Screening libraries with gene probes
Screening expression libraries with antibodies
Subcloning
Characterization of plasmid clones
Polymerase chain reaction
The PCR reaction
PCR in practice
Cloning PCR products
Long-range PCR
Reverse-transcription PCR
Rapid amplification of cDNA ends
Quantitative PCR
Applications of PCR
Characterization of a cloned gene
DNA sequencing
Databank entries and annotation
Sequence analysis
Sequence comparisons
Protein structure
Confirming gene function
Analysis of gene expression
Analysing transcription
Methods for studying the promoter
Regulatory elements and DNA-binding proteins
Translational analysis
Products from native and manipulated cloned genes
Factors affecting expression of cloned genes
Expression of cloned genes in bacteria
Expression in eukaryotic host cells
Adding tags and signals
In vitro mutagenesis
Vaccines
Genomic analysis
Genome sequencing
Analysis and annotation
Comparing genomes
Genome browsers
Relating genes and functions: genetic and physical maps
Transposon mutagenesis and other screening techniques
Conclusion
Analysis of genetic variation
Single nucleotide polymorphisms
Larger-scale variations
Other methods for studying variation
Human genetic diseases
Molecular phylogeny
Post-genomic analysis
Analysing transcription; transcriptomes
Array-based methods
Translational analysis; proteomics
Post-translational analysis: protein interactions
Integrative studies; systems biology
Modifying organisms; transgenics
Modification of bacteria and viruses: live vaccines
Transgenesis and cloning
Animal transgenesis
Applications of transgenic animals
Transgenic plants and their applications
Glossary
Bibliography
Index
Table of Contents provided by Publisher. All Rights Reserved.

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