Presents the proceedings of a conference cosponsored by the Colorado Advanced Technology Institute and the National Institute of Standards and Technology. Reviews recent advances that are expanding the envelope of bioprocessing technology, whether by technology pull or by market push. 32 chapters, each prepared by a conference speaker, address recent notable advances in six major areas: protein and enzyme engineering, biosensors and in situ measurement systems, cell-culture systems, bioreactor engineering and control, bioseparations scaleup, and emerging technologies in bioseparations. A coherent interdisciplinary update covering a far broader range of topics than any previous volume on bioprocessing.

Frontiers in Bioprocessing: An Assessment
Protein and Enzyme Engineering: The Role of Protein Crystals in Biotechnologyand Industry
Center for Advanced Research in Biotechnology: A Multidisciplinary Approach to
Protein Engineering
Cell-Free Synthesis Bioreactor
Recombination in Replicating RNAs
Enantioselective Enzymatic Synthesis of Prostaglandin-Synthons in Multiphase
Reaction Media
Design and Synthesis of a Peptide Having Chymotrypsin-Like Catalytic Activity
Biosensors and In Situ Measurement Systems: Fiber-Optic Sensors Using Raman and
Surface-Enhanced Raman Spectroscopy
Non-Invasive Spectroscopic Monitoring of a Bioprocess
In Situ On-Line Optical Fiber Sensor for Fluorescence Monitoring in Bioreactors
Fiber-Optic Biosensors Incorporating Sustained Release of Reagents
Whole Cell Biosensors: A Brief Overview and Presentation of a Novel Neuron-Based
Approach
Investigation of Immobilized-Cell Energetics and Metabolism Using NMRSpectroscopy
Cell-Culture Systems: The Formation of b-Lactamase Inclusion Bodies in
Escherichia coli and Comparison with In Vitro Studies
Secretion of Heterologous Proteins and Peptides by Pichia pastoris
Streptomyces Bioprocessing: From Secondary Metabolites to Heterologous Proteins
Strategy for the Production and Stabilization of Lignin Peroxidase from
Phanerochaete chrysosporium in Air-Lift and Stirred Tank Bioreactors
Bioprocessing of Higher Plant-Cell Cultures
The Oligosaccharides of Glycoproteins: Factors Affecting Their Synthesis and
Their Influence on Glycoprotein Properties
Modeling the Regulation of Antibody Synthesis and Assembly in a Murine Hybridoma
Bioreactor Engineering and Control: Modeling of Immobilized Microbial Cells:Influence of Substrate or Product Inhibition on Activity and Biomass Spatial
Profiles
Micromixing in Fermentation-The Response of Microorganisms to Hydrodynamic
Forces
Gas-Liquid Oxygen Transfer in Perfluorochemical-in-Water Dispersions
Strategies to Minimize Fouling in the Membrane Processing of Biofluids
Bioseparation Scale-up: Role of Functionalized Membrane Separation Technology in
Downstream Processing of Biotechnology-Derived Proteins
Extraction of Lipases in Aqueous Two-Phase Systems: The Strategy for an
Optimization
Salt-Polyethylene Glycol Two-Phase Aqueous Systems to Purify Proteins and
Nucleic Acid Mixtures
A Polyethylene Glycol-Sodium Chloride Multiphase System for Extraction of Acid
Hydrolysates
Emerging Technologies in Bioseparations: An Expert System for Selection and
Synthesis of Protein Purification Processes
Thermodynamics of Aqueous Mixtures of Salts and Polymers
Membrane Affinity Technology for Biomolecule Purification and Diagnostics
Recycling Free-Flow Focusing: Apparatus and Applications
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