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Preface | p. v |
Contributors | p. xiii |
Introduction | |
A Brief Summary of the Different Types of Mass Spectrometers Used in Proteomics | p. 3 |
Experimental Setups and Considerations to Study Microbial Interactions | p. 17 |
Proteomics | |
Plant Proteomics | p. 29 |
Methods for Human CD8+ T Lymphocyte Proteome Analysis | p. 45 |
Label-Free Proteomics of Serum | p. 67 |
Flow Cytometric Analysis of Cell Membrane Microparticles | p. 79 |
Protein Expression Profiling | |
Exosomes | p. 97 |
Toward a Full Characterization of the Human 20S Proteasome Subunits and Their Isoforms by a Combination of Proteomic Approaches | p. 111 |
Free-Flow Electrophoresis of the Human Urinary Proteome | p. 131 |
Versatile Screening for Binary Protein-Protein Interactions by Yeast Two-Hybrid Mating | p. 145 |
Native Fractionation lsolation of Native Membrane-Bound Protein Complexes from Porcine Rod Outer Segments Using Isopycnic Density Gradient Centrifugation | p. 161 |
Mapping of Signaling Pathways by Functional Interaction Proteomics | p. 177 |
Selection of Recombinant Antibodies by Eukaryotic Ribosome Display | p. 193 |
Production of Protein Arrays by Cell-Free Systems | p. 207 |
Nondenaturing Mass Spectrometry to Study Noncovalent Protein/Protein and Protein/Ligand Complexes: Technical Aspects and Application to the Determination of Binding Stoichiometrics | p. 217 |
Protein Processing Characterized by a Gel-Free Proteomics Approach | p. 245 |
Identification and Characterization of N-Glycosylated Proteins Using Proteomics | p. 263 |
Protein Analysis | |
Data Standards and Controlled Vocabularies for Proteomics | p. 279 |
The Pride Proteomics Identifications Database: Data Submission, Query, and Dataset Comparison | p. 287 |
Searching the Protein Interaction Space Through the Mint Database | p. 305 |
PepSeeker: Mining Information from Proteomic Data | p. 319 |
Toward High-Throughput and Reliable Peptide Identification via Ms/Ms Spectra | p. 333 |
MassSorter: Peptide Mass Fingerprinting Data Analysis | p. 345 |
Database Similarity Searches | p. 361 |
Protein Multiple Sequence Alignment | p. 379 |
Discovering Biomedical Knowledge from the Literature | p. 415 |
Protein Subcellular Localization Prediction Using Artificial Intelligence Technology | p. 435 |
Protein Functional Annotation by Homology | p. 465 |
Designability and Disease | p. 491 |
Prism: Protein-Protein Interaction Prediction by Structural Matching | p. 505 |
Prediction of Protein Interaction Based on Similarity of Phylogenetic Trees | p. 523 |
Large Multiprotein Structures Modeling and Simulation: The Need for Mesoscopic Models | p. 537 |
Dynamic Pathway Modeling of Signal Transduction Networks: A Domain-Oriented Approach | p. 559 |
Index | p. 579 |
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