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9780471253914

Fundamentals of Light Microscopy and Electronic Imaging

by
  • ISBN13:

    9780471253914

  • ISBN10:

    047125391X

  • Edition: 1st
  • Format: Hardcover
  • Copyright: 2001-10-29
  • Publisher: Wiley-Liss

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Summary

This book provides a coherent introduction to the principles and applications of the integrated electron microscope system. It is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.

Table of Contents

Preface.
Fundamentals of Light Microscopy.
Light and Color.
Illuminators, Filters, and Isolation of Specific Wavelengths.
Lenses and Geometrical Optics.
Diffraction and Interference in Image Formation.
Diffraction and Spatial Resolution.
Phase Contrast Microscopy and Dark-Field Microscopy.
Properties of Polarized Light.
Polarization Microscopy.
Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy.
Fluorescence Microscopy.
Confocal Laser Scanning Microscopy.
Video Microscopy.
Digital CCD Microscopy.
Digital Image Processing.
Image Processing for Scientific Publication.
Appendix I.
Appendix II.
Appendix III.
Glossary.
References.
Index

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