Gel Electrophoresis of Proteins A Practical Approach

by Hames, B. D.

• ISBN13: 9780199636402
• ISBN10: 0199636400
• Edition: 3rd
• Format: Paperback
• Copyright: 1998-12-10
• Publisher: Oxford University Press

Summary

This new edition of Gel Electrophoresis of Proteins is a completely new text, with eight of the ten chapters written by new authors. It presents the best methods, hints and tips for core procedures such as one- dimensional polyacrylamide gel electrophoresis, isoelectric focusing,two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements and updates of the procedures. In addition, it describes major new techniques which have come to the fore since the previous edition. Thus there are chapters on capillarygel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein:protein interactions by gel electrophoresis. One thing has not changed. The emphasis is still on describing the best methods, in step-by-step detail, with copiousadvice to ensure that each method works first time in the reader's hands. The first two editions of Gel Electrophoresis of Proteins: A Practical Approach each gained a strong reputation as easy-to-follow laboratory manuals written by experienced researchers for researchers. The methods werepresented in a clear accessible format and had been fully tested to ensure success in the lab. This new edition will strengthen the reputation of the book still further. It is a 'must have' for all those who currently use gel electrophoresis or who plan to do so.

Table of Contents

One-dimensional PAGE

Protein detection

Preparative gel electrophoresis

Capillary SDS electrophoresis

Conventional isoelectric focusing

2-D gel electrophoresis

Peptide mapping

Sequence analysis of gel-resolved proteins

Analysis of glycoproteins

Protein: protein interactions

List of suppliers

Bibliography of polypeptide detection methods

Table of Contents provided by Publisher. All Rights Reserved.