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Gene Cloning and DNA Analysis: An Introduction

by Brown, T. A.

ISBN13: 9780632059010
ISBN10: 063205901X
Edition: 4th
Format: Paperback
Copyright: 2001-10-01
Publisher: John Wiley & Sons Inc

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1. Gene Cloning and DNA Analysis: An Introduction > ISBN13: 9780632059010

List Price: $68.95

Summary

The fourth edition of Gene Cloning is a fully revised and updated version of this most popular introductory text on the subject. This exciting new edition retains the basic structure and organisation that has proved successful for the previous three editions.Two major changes in the contents of the book are the introduction of the subject of the polymerase chain reaction (PCR) at the beginning of the book with more emphasis being placed on PCR throughout and the inclusion of a new chapter on genome sequencing and functional analysis.

Author Biography

Terry Brown is Professor of Biomolecular Archaeology at the Department of Biomolecular Sciences, UMIST, Manchester, UK

Preface to the Fourth Edition

vii

Preface to the Third Edition

Preface to the Second Edition

Preface to the First Edition

xii

Part 1: The Basic Principles of Gene Cloning and DNA Analysis

(194)

Why Gene Cloning and DNA Analysis are Important

(11)

Vehicles for Gene Cloning: Plasmids and Bacteriophages

(14)

Purification of DNA from Living Cells

(24)

Manipulation of Purified DNA

(33)

Introduction of DNA into Living Cells

(19)

Cloning Vectors for E. coli

104

(26)

Cloning Vectors for Eukaryotes

130

(26)

How to Obtain a Clone of a Specific Gene

156

(23)

The Polymerase Chain Reaction

179

(16)

Part 2: The Applications of Gene Cloning and DNA Analysis in Research

195

(74)

Studying Gene Location and Structure

197

(20)

Studying Gene Expression and Function

217

(29)

Studying Genomes

246

(23)

Part 3: The Applications of Gene Cloning and DNA Analysis in Biotechnology

269

(72)

Production of Protein from Cloned Genes

271

(22)

Gene Cloning and DNA Analysis in Medicine

293

(20)

Gene Cloning and DNA Analysis in Agriculture

313

(16)

Gene Cloning and DNA Analysis in Forensic Science

329

(12)

Glossary

341

(16)

Index

357

Supplemental Materials

What is included with this book?

The New copy of this book will include any supplemental materials advertised. Please check the title of the book to determine if it should include any access cards, study guides, lab manuals, CDs, etc.

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5% off 1 book, 7% off 2 books, 10% off 3+ books

Gene Cloning and DNA Analysis: An Introduction

Summary

Author Biography

Table of Contents

Supplemental Materials

Rewards Program