Gene Cloning and DNA Analysis : An Introduction

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  • Edition: 6th
  • Format: Paperback
  • Copyright: 2010-04-12
  • Publisher: Wiley-Blackwell
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Known as the standard introductory text to this exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, the text carefully lays out the principles of the techniques used and their applications, using over 250 four-color illustrations. In addition to changes throughout, the final four chapters have been significantly updated and extended to reflect striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology.

Author Biography

Terry Brown, University of Manchester, Faculty of Life Sciences, UK

Table of Contents

Preface to the Sixth Edition
The Basic Principles of Gene Cloning and DNA Analysis
Why Gene Cloning and DNA Analysis are Important
The early development of genetics
The advent of gene cloning and the polymerase chain reaction
What is gene cloning?
What is PCR?
Why gene cloning and PCR are so important
How to find your way through this book
Vectors for Gene Cloning: Plasmids and Bacteriophages
Purification of DNA from Living Cells
Preparation of total cell DNA
Preparation of plasmid DNA
Preparation of bacteriophage DNA
Manipulation of Purified DNA
The range of DNA manipulative enzymes
Enzymes for cutting DNA - restriction endonucleases
Ligation - joining DNA molecules together
Introduction of DNA into Living Cells
Transformation - the uptake of DNA by bacterial cells
Identification of recombinants
Introduction of phage DNA into bacterial cells
Identification of recombinant phages
Introduction of DNA into non-bacterial cells
Cloning Vectors for E. coli
Cloning vectors based on E. coli plasmids
Cloning vectors based on M13 bacteriophage
Cloning vectors based on l bacteriophage
and other high capacity vectors enable genomic libraries to be constructed
Vectors for other bacteria
Cloning Vectors for Eukaryotes
Vectors for yeast and other fungi
Cloning vectors for higher plants
Cloning vectors for animals
How to Obtain a Clone of a Specific Gene
The problem of selection
Direct selection
Identification of a clone from a gene library
Methods for clone identification
The Polymerase Chain Reaction
The polymerase chain reaction in outline
PCR in more detail
After the PCR: studying PCR products
Real time PCR enables the amount of starting material to be quantified
The Applications of Gene Cloning and DNA Analysis in Research
Sequencing Genes and Genomes
The methodology for DNA sequencing
How to sequence a genome
Studying Gene Expression and Function
Studying the RNA transcript of a gene
Studying the regulation of gene expression
Identifying and studying the translation product of a cloned gene
Studying Genomes
Genome annotation
Studies of the transcriptome and proteome
The Applications of Gene Cloning and DNA Analysis in Biotechnology
Production of Protein from Cloned Genes
Special vectors for expression of foreign genes in E. coli
General problems with the production of recombinant protein in E. coli
Production of recombinant protein by eukaryotic cells
Gene Cloning and DNA Analysis in Medicine
Production of recombinant pharmaceuticals
Identification of genes responsible for human diseases
Gene therapy
Gene Cloning and DNA Analysis in Agriculture
The gene addition approach to plant genetic engineering
Gene subtraction
Problems with genetically modified plants
Gene Cloning and DNA Analysis in Forensic Science and Archaeology
DNA analysis in the identification of crime suspects
Studying kinship by DNA profiling
Sex identification by DNA analysis
Archaeogenetics - using DNA to study human prehistory
Table of Contents provided by Publisher. All Rights Reserved.

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