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| Preface to the Sixth Edition | |
| The Basic Principles of Gene Cloning and DNA Analysis | |
| Why Gene Cloning and DNA Analysis are Important | |
| The early development of genetics | |
| The advent of gene cloning and the polymerase chain reaction | |
| What is gene cloning? | |
| What is PCR? | |
| Why gene cloning and PCR are so important | |
| How to find your way through this book | |
| Vectors for Gene Cloning: Plasmids and Bacteriophages | |
| Plasmids | |
| Bacteriophages | |
| Purification of DNA from Living Cells | |
| Preparation of total cell DNA | |
| Preparation of plasmid DNA | |
| Preparation of bacteriophage DNA | |
| Manipulation of Purified DNA | |
| The range of DNA manipulative enzymes | |
| Enzymes for cutting DNA - restriction endonucleases | |
| Ligation - joining DNA molecules together | |
| Introduction of DNA into Living Cells | |
| Transformation - the uptake of DNA by bacterial cells | |
| Identification of recombinants | |
| Introduction of phage DNA into bacterial cells | |
| Identification of recombinant phages | |
| Introduction of DNA into non-bacterial cells | |
| Cloning Vectors for E. coli | |
| Cloning vectors based on E. coli plasmids | |
| Cloning vectors based on M13 bacteriophage | |
| Cloning vectors based on l bacteriophage | |
| ¿ and other high capacity vectors enable genomic libraries to be constructed | |
| Vectors for other bacteria | |
| Cloning Vectors for Eukaryotes | |
| Vectors for yeast and other fungi | |
| Cloning vectors for higher plants | |
| Cloning vectors for animals | |
| How to Obtain a Clone of a Specific Gene | |
| The problem of selection | |
| Direct selection | |
| Identification of a clone from a gene library | |
| Methods for clone identification | |
| The Polymerase Chain Reaction | |
| The polymerase chain reaction in outline | |
| PCR in more detail | |
| After the PCR: studying PCR products | |
| Real time PCR enables the amount of starting material to be quantified | |
| The Applications of Gene Cloning and DNA Analysis in Research | |
| Sequencing Genes and Genomes | |
| The methodology for DNA sequencing | |
| How to sequence a genome | |
| Studying Gene Expression and Function | |
| Studying the RNA transcript of a gene | |
| Studying the regulation of gene expression | |
| Identifying and studying the translation product of a cloned gene | |
| Studying Genomes | |
| Genome annotation | |
| Studies of the transcriptome and proteome | |
| The Applications of Gene Cloning and DNA Analysis in Biotechnology | |
| Production of Protein from Cloned Genes | |
| Special vectors for expression of foreign genes in E. coli | |
| General problems with the production of recombinant protein in E. coli | |
| Production of recombinant protein by eukaryotic cells | |
| Gene Cloning and DNA Analysis in Medicine | |
| Production of recombinant pharmaceuticals | |
| Identification of genes responsible for human diseases | |
| Gene therapy | |
| Gene Cloning and DNA Analysis in Agriculture | |
| The gene addition approach to plant genetic engineering | |
| Gene subtraction | |
| Problems with genetically modified plants | |
| Gene Cloning and DNA Analysis in Forensic Science and Archaeology | |
| DNA analysis in the identification of crime suspects | |
| Studying kinship by DNA profiling | |
| Sex identification by DNA analysis | |
| Archaeogenetics - using DNA to study human prehistory | |
| Glossary | |
| Index | |
| Table of Contents provided by Publisher. All Rights Reserved. |
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