Gene Probes 2 A Practical Approach

by Hames, B. D.; Higgins, S. J.

• ISBN13: 9780199634149
• ISBN10: 0199634149
• Format: Paperback
• Copyright: 1996-01-18
• Publisher: IRL Press

Summary

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. The advent of solid supports, new DNA labeling techniques, and PCR technology has created a need for an up-to-date practical manual in this area. Gene Probes (Applications), describes techniques for specific research goals. It covers: hybridization strategies; choosing probes; screening and analysis of DNA libraries, including the use of PCR; blotting techniques, gene targeting; and analysis of human genetic mutations. Its companion volume, Gene Probes 1, describes the wide rage of techniques now available for the purification, synthesis, labeling, detection, and quantitation of probes.

Table of Contents

Hybridization strategy

Screening recombinant DNA libraries by hybridization and amplification

Screening human recombinant libraries with nucleic acid probes

Antibody screening of bacterial expression systems

Isolation of cDNA clones by direct expression in mammalian cells

Genomic mapping and gene structure analysis

Analysis of gene expression by blotting techniques

In situ hybridization of nucleic acid probes to cellular RNA

Applications of chromosomal in situ hybridization

Analysis of gene expression analysis by PCR: a quantitative approach

Analysis of protein expression by immunocytochemistry

Gene targeting in mammalian cells

Directed mutagenesis and mutant analysis

Human gene mutational analysis

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