Gene Therapy of Cancer

by Walther, Wolfgang; Stein, Ulrike

• ISBN13: 9781934115855
• ISBN10: 1934115851
• Edition: 2nd
• Format: Hardcover
• Copyright: 2009-07-30
• Publisher: Humana Pr Inc

Summary

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. "Gene Therapy of Cancer: Methods and Protocols, Second Edition" fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Cutting edge and authoritative, "Gene Therapy of Cancer: Methods and Protocols, Second Edition" is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.

Table of Contents

Preface	p. v
Contributors	p. xi
Experimental Approaches in Cancer Therapy
Introduction
The Development of Gene Therapy: From Monogenic Recessive Disorders to Complex Diseases Such as Cancer
Vectorology For Cancer Gene Therapy
Designing Adenoviral Vectors for Tumor-Specific Targeting	p. 57
Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures	p. 75
Use of Minicircle Plasmids for Gene Therapy	p. 87
Transposable Elements as Plasmid-Based Vectors for Long-Term Gene Transfer into Tumors	p. 105
Designing Plasmid Vectors	p. 117
Development of Bacterial Vectors for Tumor-Targeted Gene Therapy	p. 131
Nonviral Transfer Technologies in Cancer Gene Therapy
Electroporative Gene Transfer	p. 157
Gene Gun Delivery Systems for Cancer Vaccine Approaches	p. 167
Ultrasound-Mediated Gene Transfection	p. 179
Nonviral Jet-Injection Technology for Intratumoral In Vivo Gene Transfer of Naked DNA	p. 195
Experimental Studies In Cancer Gene Therapy
Methods for Constructing and Evaluating Antitumor DNA Vaccines	p. 211
Immunity of Lentiviral Vector-Modified Dendritic Cells	p. 245
Saporin Suicide Gene Therapy	p. 261
Using In Vivo Biopanning for the Development of Radiation-Guided Drug Delivery Systems	p. 285
Chemosensitization of Tumor Cells: Inactivation of Nuclear Factor-Kappa B Associated with Chemosensitivity in Mealanoma Cels After Combination Treatment with E2F-1 and Doxorubicin	p. 301
Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy	p. 315
Silencing Epidermal Growth Factor Receptor by RNA Interference in Glioma	p. 335
Delivery of Phosphorodiamidate Morpholino Antisense Oligomers in Cancer Cells	p. 351
Use of RNA Aptamers for the Modulation of Cancer Cell Signaling	p. 363
G-Rich Oligonucleotides for Cancer Treatment	p. 379
Clinical Approaches in Cancer Gene Therapy
Requirements for Clinical Gene Therapy Trials
Regulatory Aspects for Translating Gene Therapy Research into the Clinic	p. 397
Ethics of Cancer Gene Transfer Clinical Research	p. 423
Virus Production for Clinical Gene Therapy	p. 447
Production of Plasmid DNA as Pharmaceutical	p. 471
Clinical Protocols in Cancer Gene Therapy
Gene Immunotherapy for Non-Small Cell Lung Cancer	p. 499
Gene Therapy for Antitumor Vaccination	p. 515
HSV-tk/IL-2 Gene Therapy for Glioblastoma Multiforme	p. 529
Construction and Characterization of an Oncolytic HSV Vector Containing a Fusogenic Glycoprotein and Prodrug Activation for Enhanced Local Tumor Control	p. 551
Newcastle Disease Virus: A Promising Vector for Viral Therapy, Immune Therapy, and Gene Therapy of Cancer	p. 565
Oncolytic Viral Therapy Using Reovirus	p. 607
Design and Testing of Novel Oncolytic Vaccinia Strains	p. 635
Tumor-Targeted Salmonella typhimurium Overexpressing Cytosine Deaminase: A Novel, Tumor-Selective Therapy	p. 649
Chemoprotection by Transfer of Resistance Genes	p. 661
Phase I Clinical Trial of Locoregional Administration of the Oncolytic Adenovirus ONYX-015 in Combination with Mitomycin-C, Doxorubicin, and Cisplatin Chemotherapy in Patients with Advanced Sarcomas	p. 705
Index	p. 719
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