Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual

by Friedmann, Theodore; Rossi, John

• ISBN13: 9780879697655
• ISBN10: 0879697652
• Edition: 1st
• Format: Paperback
• Copyright: 2006-11-10
• Publisher: Cold Spring Harbor Laboratory Press

Summary

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNAprovides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.

Table of Contents

Introduction	1	(2)
Theodore Friedmann
John Rossi
VIRAL VECTORS
Retroviral Vectors	3	(20)
Kenneth Cornetta
Karen E. Pollok
A. Dusty Miller
Development of Lentiviral Vectors Expressing siRNA	23	(12)
Gustavo Tiscornia
Oded Singer
Inder M. Verma
HIV-2 Vectors in Human Gene Therapy: Design, Construction, and Therapuetic Potential	35	(12)
Kevin V. Morris
Flossie Wong-Staal
SIV Vectors as Vehicles for DNA Delivery	47	(10)
Els Verhoeyen
Francois-Loic Cosset
Didier Negre
Production and Use of Feline Immunodeficiency Virus-based Lentiviral Vectors	57	(18)
Dyana T. Saenz
Roman Barraza
Nils Loewen
Wulin Teo
Eric M. Poeschla
Lentivirus Transduction of Hematopoietic Cells	75	(8)
Ming-Jie Li
John J. Rossi
Spleen Necrosis Virus-based Vectors	83	(8)
Zahida Parveen
Muhammad Mukhtar
Roger J. Pomerantz
Foamy Virus Vector Production and Transduction of Hematopoietic Cells	91	(8)
Neil C. Josephson
David W. Russell
Simian Foamy Virus Type-1 Vectors	99	(8)
Jeonghae Park
Ayalew Mergia
Generation of VSV-G-pseudotyped Retroviral Vectors	107	(12)
Jiing-Kuan Yee
Targeted Gene Transfer with Surface-engineered Lentiviral Vectors	119	(12)
Els Verhoeyen
Francois-Loic Cosset
Preparation of Pseudotyped Lentiviral Vectors Resistant to Inactivation by Serum Complement	131	(6)
Ghiabe H. Guibinga
Theodore Friedmann
Generation of 2A Peptide-linked Multicistronic Vectors	137	(12)
Andrea L. Szymczak-Workman
Kate M. Vignali
Dario A.A. Vignali
Construction of First-generation Adenoviral Vectors	149	(18)
P. Joel Ross
Robin J. Parks
Production and Characterization of Helper-dependent Adenoviral Vectors	167	(16)
Donna J. Palmer
Philip Ng
Cell and Tissue Targeting	183	(14)
Yosuke Kawakami
David T. Curiel
Stable Producer Cell Lines for AAV Assembly	197	(6)
Gilliane Chadeuf
Anna Salvetti
Strategies for the Design of Hybrid Adeno-associated Virus Vectors	203	(14)
Aravind Asokan
R. Jude Samulski
Recombinant Herpes Simplex Virus Vectors	217	(10)
William F. Goins
David M. Krisky
James B. Wechuck
Darren Wolfe
Shaohua Huang
Joseph C. Glorioso
Herpes Simplex Virus Type-1-derived Amplicon Vectors	227	(28)
William J. Bowers
Howard J. Federoff
γ-2 Herpesvirus Saimiri-based Vectors	255	(10)
Adrian Whitehouse
Gene Delivery Using HSV/AAV Hybrid Amplicon Vectors	265	(8)
Okay Saydam
Daniel L. Glauser
Cornel Fraefel
Polyomaviruses: SV40	273	(16)
David S. Strayer
Christine Mitchell
Dawn A. Maier
Carmen N. Nichols
SV40 In Vitro Packaging: A Pseudovirion Gene Delivery System	289	(8)
Chava Kimchi-Sarfaty
Michael M. Gottesman
Baculovirus-based Display and Gene Delivery Systems	297	(16)
Anna R. Makela
Wolfgang Ernst
Reingard Grabherr
Christian Oker-Blom
Safe, Simple, and High-capacity Gene Delivery into Insect and Vertebrate Cells by Recombinant Baculoviruses	313	(14)
Kari J. Airenne
Olli H. Laitinen
Anssi J. Mahonen
Seppo Yla-Herttuala
Alphaviruses: Semliki Forest Virus and Sindbis Virus as Gene Delivery Vectors	327	(22)
Kenneth Lundstrom
Gene Transfer into Mammalian Cells Using Targeted Filamentous Bacteriophage	349	(10)
Andrew Baird
Selection, Isolation, and Identification of Targeting Peptides for Ligand-directed Gene Delivery	359	(12)
Martin Trepel
Wadih Arap
Renata Pasqualini
Rescue and Propagation of Tropism-modified Measles Viruses	371	(10)
Takafumi Nakamura
Stephen J. Russell
Picornavirus-based Expression Vectors	381	(8)
Steffen Mueller
Eckard Wimmer
Reverse Genetics of Influenza Viruses	389	(6)
Glenn A. Marsh
Peter Palese
NONVIRAL TECHNIQUES AND VECTORS
An Overview of Condensing and Noncondensing Polymeric Systems for Gene Delivery	395	(10)
Dinesh B. Shenoy
Mansoor M. Amiji
Transfection of Hippocampal Neurons with Plasmid DNA Using Calcium Phosphate Coprecipitation	405	(6)
Bernhard Goetze
Michael Kiebler
Gene Delivery to Skin Using Biolistics	411	(8)
William C. Heiser
Optimizing Electrotransfection of Mammalian Cells In Vitro	419	(8)
Shulin Li
Micro In Utero Electroporation for Efficient Gene Targeting in Mouse Embryos	427	(6)
Tomomi Shimogori
Lipoplex and LPD Nanoparticles for In Vivo Gene Delivery	433	(8)
Shyh-Dar Li
Song Li
Leaf Huang
Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery	441	(10)
Weijun Li
Francis C. Szoka, Jr.
HVJ Liposomes and HVJ Envelope Vectors	451	(10)
Yasufumi Kaneda
Polylysine Copolymers for Gene Delivery	461	(12)
Sung Wan Kim
PEI Nanoparticles for Targeted Gene Delivery	473	(6)
Frank Alexis
Jieming Zeng
Shu Wang
Cyclodextrin-containing Polycations for Nucleic Acid Delivery	479	(8)
Jeremy D. Heidel
Bionanocapsules Using the Hepatitis B Virus Envelope L Protein	487	(4)
Tadanori Yamada
Joohee Jung
Masaharu Seno
Akihiko Kondo
Masakazu Ueda
Katsuyuki Tanizawa
Shun'ichi Kuroda
Formulations of Solid Lipid Nanoparticles for Transfection of Mammalian Cells In Vitro	491	(6)
Carsten Rudolph
Joseph Rosenecker
PEGylated Poly-L-lysine DNA Nanoparticles	497	(4)
Pamela B. Davis
Tomasz H. Kowalczyk
Water-soluble Lipopolymers and Lipopeptides for Nucleic Acid Delivery	501	(6)
Ram I. Mahato
Zhaoyang Ye
Sung Wan Kim
Cationic Polysaccharides for DNA Delivery	507	(8)
Ira Yudovin-Farber
Hagit Eliyahu
Abraham J. Domb
Sustained Release of Plasmid DNAs Encoding Platelet-derived Growth Factor and Hyaluronan Synthase 2 from Cross-linked Hyaluronan Matrices and Films	515	(6)
Weiliam Chen
Linear Polyethylenimine: Synthesis and Transfection Procedures for In Vitro and In Vivo	521	(6)
Manfred Ogris
Ernst Wagner
Protein Nanospheres for Gene Delivery: Preparation and In Vitro Transfection Studies with Gelatin Nanoparticles	527	(14)
Sushma Kommareddy
Mansoor M. Amiji
Vesicular Stomatitis Virus-G Conjugate	541	(6)
Atsushi Miyanohara
High-throughput Methods for Screening Polymeric Transfection Reagents	547	(8)
Gregory T. Zugates
Daniel G. Anderson
Robert Langer
Poly(Lactic Acid) and Poly(Ethylene Oxide) Nanoparticles as Carriers for Gene Delivery	555	(6)
Noemi S. Csaba
Alejandro Sanchez
Maria Jose Alonso
Biodegradable Nanoparticles	561	(6)
Jaspreet K. Vasir
Vinod Labhasetwar
Transposon-mediated Delivery of Small Interfering RNA: Sleeping Beauty Transposon	567	(6)
Bradley S. Fletcher
Efficient DNA Delivery into Mammalian Cells by Displaying the TAT Transduction Domain on Bacteriophage λ	573	(8)
Jehangir Wadia
Akiko Eguchi
Steven F. Dowdy
Cell-penetrating Peptide-mediated Delivery of Peptide Nucleic Acid Oligomers	581	(6)
Pontus Lundberg
Kalle Kilk
Ulo Langel
REGULATION OF TRANSGENE EXPRESSION
Conditional Mutagenesis of the Genome Using Site-specific DNA Recombination	587	(16)
Kazuaki Ohtsubo
Jamey D. Marth
Expression and Validation of Ribozyme and Short Hairpin RNA in Mammalian Cells	603	(12)
Mohammed Amarzguioui
Mifepristone-inducible Gene Regulatory System	615	(16)
Kurt Schillinger
Xiangcang Ye
Sophia Tsai
Bert W. O'Malley
Dimerizer-mediated Regulation of Gene Expression	631	(12)
Victor M. Rivera
Lori Berk
Tim Clackson
RheoSwitch System: A Highly Sensitive Ecdysone Receptor-based Gene Regulation System Induced by Synthetic Small-molecule Ligands	643	(10)
Prasanna Kumar
Anand Katakam
Site-specific Integration with Phage φC31 Integrase	653	(8)
R. Tyler Hillman
Michele P. Calos
Creating Zinc Finger Nucleases to Manipulate the Genome in a Site-specific Manner Using a Modular-assembly Approach	661	(14)
Matthew Porteus
SPECIALIZED TECHNIQUES OF GENE AND VECTOR DELIVERY
Assembly of De Novo Bacterial Artificial Chromosome-based Human Artificial Chromosomes	675	(16)
Joydeep Basu
Huntington F. Willard
Delivery of Naked DNA Using Hydrodynamic Injection Techniques	691	(10)
David L. Lewis
Mark Noble
Julia Hegge
Jon Wolff
Nonviral Gene Transfer across the Blood-brain Barrier with Trojan Horse Liposomes	701	(10)
William M. Pardridge
Sonoporation: An Efficient Technique for the Introduction of Genes into Chick Embryos	711	(6)
Sho Ohta
Ogino Yukiko
Kentro Suzuki
Mika Kamimura
Katsuro Tachibana
Gen Yamada
Genetic Manipulation of Mammalian Cells by Microinjection	717	(6)
David W. Rose
Magnetofection	723	(8)
Christian Plank
Joseph Rosenecker
Photochemical Internalization for Light-directed Gene Delivery	731	(8)
Anette Bonsted
Anders Hogset
Ernst Wagner
Kristian Berg
TRANSGENIC APPROACHES
Pronuclear Microinjection in Mice	739	(16)
Walter Tsark
Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors	755	(6)
Oded Singer
Gustavo Tiscornia
Inder M. Verma
Appendix: Cautions	761	(10)
Subject Index	771