(BIOS Scientific Publishers) Glaxo Wellcome Research and Development, Stevenage, UK. Contains a current summary of how best to genetically engineer viruses. Includes an overview of basic virology. For virologists and molecular biologists. Covers prokaryotic systems, insect and plant systems, therapeutic applications, and ethical and regulatory issues. Softcover.

Contributors

vii

Abbreviations

viii

Preface

xi

Introduction: Viruses as vehicles and expressors of genetic material.

1

(4)

C.J.A. Ring

E.D. Blair

Prokaryotic viruses.

5

(38)

M.D. Jones

Introduction

5

(1)

Bacteriophage λ vectors

6

(5)

Filamentous bacteriophage vectors

11

(10)

Phage display systems

21

(10)

Phage gene delivery vectors

31

(3)

Bacteriophages as antibiotics

34

(1)

Conclusion

35

(1)

References

35

(8)

Insect viruses.

43

(16)

I.M. Jones

Introduction

43

(2)

The baculovirus expression system

45

(9)

References

54

(5)

Plant viruses.

59

(48)

C. Lacomme

G.P. Pogue

T.M.A. Wilson

S. Santa Cruz

Introduction

59

(1)

General properties of plant viruses

60

(4)

Development of plant virus-based vectors

64

(12)

Applications of plant virus-based vectors in basic research

76

(12)

Commercial applications of plant virus-based vectors

88

(10)

Conclusions

98

(1)

References

99

(8)

Mammalian expression systems and vaccination.

107

(52)

M.W. Carroll

G.W.G. Wilkinson

K. Lundstrom

Introduction

107

(1)

Recombinant poxvirus expression vectors

108

(12)

Recombinant adenovirus expression vectors

120

(13)

Recombinant alphavirus expression vectors

133

(8)

Conclusions

141

(1)

References

142

(17)

Genetic engineering of animal DNA viruses in order to study and modify pathogenesis.

159

(28)

D.A. Leib

Introduction

159

(1)

Reverse genetics and animal models

160

(1)

Herpes simplex virus

161

(5)

Regulation of HSV gene expression and pathogenesis

166

(4)

Immune evasion and pathogenesis

170

(7)

Conclusions

177

(1)

References

178

(9)

Genetic engineering of animal RNA viruses in order to study and modify pathogenesis.

187

(28)

W.S. Barclay

D.J. Evans

Introduction

187

(1)

The positive-strand RNA viruses

188

(10)

The negative-strand RNA viruses

198

(8)

Concluding remarks

206

(1)

References

207

(8)

Therapeutic applications of viral vectors.

215

(40)

P. Lowenstein

D. Stone

A. David

A. Melcher

R. Vile

C. Thomas

D. Bataille

M. Castro

Viruses as vectors: what's new?

215

(5)

Retroviral vectors

220

(6)

HSV-1 vectors

226

(7)

Adenoviruses as vectors for gene therapy

233

(6)

Adeno-associated virus (AAV) vectors

239

(4)

References

243

(12)

The safety of work with genetically modified viruses.

255

(20)

S.R. Warne

Introduction

255

(1)

Physical containment

256

(4)

Experimental design and risk assessment

260

(5)

Competency of staff and safety management issues

265

(3)

The regulatory framework and ethical issues

268

(1)

Discussion and conclusions

269

(3)

References

272

(3)

Index

275

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