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Preface | p. ix |
Overview of Electroporation and Electrofusion | p. 1 |
Mechanisms and Fundamental Processes in Electroporation and Electrofusion | |
Structure and Dynamics of Electric Field-Induced Membrane Pores as Revealed by Rapid-Freezing Electron Microscopy | p. 9 |
Events of Membrane Electroporation Visualized on a Time Scale from Microsecond to Seconds | p. 29 |
Time Sequence of Molecular Events in Electroporation | p. 47 |
Electropores in Lipid Bilayers and Cell Membranes | p. 63 |
Biophysical Considerations of Membrane Electroporation | p. 77 |
Progress toward a Theoretical Model for Electroporation Mechanism: Membrane Electrical Behavior and Molecular Transport | p. 91 |
Mechanisms of Electroporation and Electrofusion | p. 119 |
Interfacial Membrane Alteration Associated with Electropermeabilization and Electrofusion | p. 139 |
Membrane Fusion Kinetics | p. 155 |
Effects of Intercellular Forces on Electrofusion | p. 167 |
Dynamics of Cytoskeletal Reorganization in CV-1 Cells during Electrofusion | p. 179 |
Applications of Electroporation and Electrofusion in Current Research | |
Gene Transfer into Adherent Cells Growing on Microbeads | p. 201 |
Gene Targeting and Electroporation | p. 209 |
Pollen Electrotransformation for Gene Transfer in Plants | p. 227 |
Electrofusion of Plant Protoplasts and the Production of Somatic Hybrids | p. 249 |
Electrotransformation of Bacteria by Plasmid DNA | p. 265 |
Creating Vast Peptide Expression Libraries: Electroporation as a Tool to Construct Plasmid Libraries of Greater than 109 Recombinants | p. 291 |
Electroporation and Electrofusion Using a Pulsed Radio-Frequency Electric Field | p. 303 |
Electroinsertion: An Electrical Method for Protein Implantation into Cell Membranes | p. 327 |
Electroporation as a Tool to Study Enzyme Activities in Situ | p. 347 |
Comparison of PEG-Induced and Electric Field-Mediated Cell Fusion in the Generation of Monoclonal Antibodies against a Variety of Soluble and Cellular Antigens | p. 363 |
Production of Genetically Identical Embryos by Electrofusion | p. 371 |
Development of Cell-Tissue Electrofusion for Biological Applications | p. 393 |
Novel Applications of Electroporation | p. 411 |
Practical Protocols for Electroporation and Electrofusion | |
Design of Protocols for Electroporation and Electrofusion: Selection of Electrical Parameters | p. 429 |
Protocols for Using Electroporation to Stably or Transiently Transfect Mammalian Cells | p. 457 |
Optimization of Electroporation Using Reporter Genes | p. 465 |
Genetic Manipulation of Plant Cells by Means of Electroporation and Electrofusion | p. 471 |
Protocols for the Transformation of Bacteria by Electroporation | p. 485 |
Protocol for High-Efficiency Yeast Transformation | p. 501 |
Protocols of Electroporation and Electrofusion for Producing Human Hybridomas | p. 507 |
Human Hybridoma Formation by Hypo-Osmolar Electrofusion | p. 523 |
Electrically Induced Fusion and Activation in Nuclear Transplant Embryos | p. 535 |
Instrumentation for Electroporation and Electrofusion | |
Pulse Generators for Electrofusion and Electroporation | p. 555 |
Index | p. 571 |
Table of Contents provided by Syndetics. All Rights Reserved. |
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