Guide to Yeast Genetics and Molecular and Cell Biology, Part B

by Unknown

• ISBN13: 9780123106711
• ISBN10: 0123106710
• Edition: 1st
• Format: Paperback
• Copyright: 2002-06-18
• Publisher: Elsevier Science

Summary

This volume and its companion Volume 351 will supplement Volume 194 of MIE. The guides are specifically designed to meet the needs of graduate students and postdocs as well as researchers. Whether an established researcher or newcomer to the field, these volumes will contain all the up-to-date methods needed to study "Genes in Yeast." Procedures are included to enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information will be given for proven procedures that can be used as a guide for developing protocols in a number of disciplines.

Table of Contents

Contributors to Volume 350	ix
Preface	xv
Volumes in Series	xvii
Section I. Basic Techniques
Getting Started with Yeast	3	(39)
Fred Sherman
How to Set up a Yeast Laboratory	42	(30)
Cora Styles
Constructing Yeast Libraries	72	(15)
Haoping Liu
Transformation of Yeast by Lithium Acetate Single-Stranded Carrier DNA/Polyethylene Glycol Method	87	(10)
R. Daniel Gietz
Robin A. Woods
Genetic Transformation of Saccharomyces cerevisiae Mitochondria	97	(15)
Nathalie Bonnefoy
Thomas D. Fox
LacZ Assays in Yeast	112	(19)
Steffen Rupp
Analysis of Budding Patterns	131	(10)
Matthew Lord
Tracy Chen
Atsushi Fujita
John Chant
Uses and Abuses of HO Endonuclease	141	(24)
James E. Haber
Assays for Gene Silencing in Yeast	165	(24)
Fred van Leeuwen
Daniel E. Gottschling
Section II. Making Mutants
Classical Mutagenesis Techniques	189	(10)
Christopher W. Lawrence
Introduction of Point Mutations into Cloned Genes	199	(20)
Brendan Cormack
Irene Castano
Insertional Mutagenesis: Transposon-Insertion Libraries as Mutagens in Yeast	219	(11)
Anuj Kumar
Susana Vidan
Michael Snyder
Tn7-Mediated Mutagenesis of Saccharomyces cerevisiae Genomic DNA in Vitro	230	(18)
Nurjana Bachman
Matthew C. Biery
Jef D. Boeke
Nancy L. Craig
Vector Systems for Heterologous Expression of Proteins in Saccharomyces cerevisiae	248	(10)
Martin Funk
Rainer Niedenthal
Dominik Mumberg
Kay Brinkmann
Volker Ronicke
Thomas Henkel
Cloning-Free Genome Alterations in Saccharomyces cerevisiae Using Adaptamer-Mediated PCR	258	(20)
Robert J. D. Reid
Michael Lisby
Rodney Rothstein
kar-Mediated Plasmid Transfer between Yeast Strains: Alternative to Traditional Transformation Methods	278	(12)
Bil yana Georgieva
Rodney Rothstein
Gene Disruption	290	(26)
Mark Johnston
Linda Riles
Johannes H. Hegemann
Synthetic Dosage Lethality	316	(13)
Vivien Measday
Philip Hieter
Section III. Genomics
Saccharomyces Genome Database	329	(18)
Laurie Issel-Tarver
Karen R. Christie
Kara Dolinski
Rey Andrada
Rama Balakrishnan
Catherine A. Ball
Gail Binkley
Stan Dong
Selina S. Dwight
Dianna G. Fisk
Midori Harris
Mark Schroeder
Anand Sethuraman
Kane Tse
Shuai Weng
David Bostein
J. Michael Cherry
Three Yeast Proteome Databases: YPD, PombePD, and CalPD (MycoPathPD)	347	(26)
Csilla Csank
Maria C. Costanzo
Jodi Hirschman
Peter Hodges
Janice E. Kranz
Mary Mangan
Kathy E. O'Neill
Laura S. Robertson
Marek S. Skrzypek
Joan Brooks
James I. Garrels
Database Resources Relevant to Yeast Biology	373	(7)
Fran Lewitter
Searching Yeast Intron Data at Ares Lab Web Site	380	(13)
Leslie Grate
Manuel Ares, Jr.
Yeast Genomic Expression Studies Using DNA Microarrays	393	(21)
Audrey P. Gasch
Transcriptome Analysis of Saccharomyces cerevisiae Using Serial Analysis of Gene Expression	414	(31)
Munira A. Basrai
Philip Hieter
PCR-Based Engineering of Yeast Genome	445	(24)
Marie E. Petracek
Mark S. Longtine
ChIP-chip: A Genomic Approach for Identifying Transcription Factor Binding Sites	469	(15)
Christine E. Horak
Michael Snyder
Computational Approaches to Identifying Transcription Factor Binding Sites in Yeast Genome	484	(15)
Hao Li
Section IV. Proteomics
Array-Based Methods for Identifying Protein-Protein and Protein-Nucleic Acid Interactions	499	(14)
Joseph F. Gera
Tony R. Hazbun
Stanley Fields
Building Protein-Protein Networks by Two-Hybrid Mating Strategy	513	(12)
Micheline Fromont-Racine
Jean-Christophe Rain
Pierre Legrain
Integrated Version of Reverse Two-Hybrid System for the Postproteomic Era	525	(21)
Hideki Endoh
Sylvie Vincent
Yves Jacob
Eleonore Real
Albertha J. M. Walhout
Marc Vidal
Biochemical Genomics Appraoch to Map Activities to Genes	546	(13)
Eric M. Phizicky
Mark R. Martzen
Stephen M. McCraith
Sherry L. Spinelli
Feng Xing
Neil P. Shull
Ceri Van Slyke
Rebecca K. Montagne
Francy M. Torres
Stanley Fields
Elizabeth J. Grayhack
Use of Two-Dimensional Gels in Yeast Proteomics	559	(26)
Anders Blomberg
Author Index	585	(24)
Subject Index	609