Handbook of Histology Methods for Bone and Cartilage

by An, Yuehuei H.; Martin, Kylie L.

• ISBN13: 9780896039605
• ISBN10: 0896039609
• Format: Hardcover
• Copyright: 2003-06-01
• Publisher: Humana Pr Inc

Summary

The first comprehensive collection of all the major methods for studying the histology and histomorphometry of bone and cartilage. The detailed methods include techniques for bone labeling, bone sectioning, histological analysis, histomorphometric analysis, radiological examination, and contact radiography. Among the applications presented are in situ hybridization of bone and cartilage, radiological examination of calcified tissue with emphasis on bone, and dealing with histology artifacts in hard tissue research. More practical than theoretical, the text is simple and straightforward, with many tables, line drawings, and micro and macro photographs to illustrate the content. Full bibliographies at the end of each chapter guide the reader to additional detailed information.

Table of Contents

Preface	p. vii
About the Editors	p. xii
Contributors	p. xiii
List of Color Plates	p. xvii
Introduction
Introduction to Experimental Bone and Cartilage Histology	p. 3
Structure and Function of Bone and Cartilage
Cell Structure and Biology of Bone and Cartilage	p. 35
Normal Structure and Function of Bone	p. 59
Structure and Function of Articular Cartilage	p. 73
Tissue Harvesting, Fixation, and Preparation
Bone-Labeling Techniques	p. 99
Human Bone Biopsy	p. 119
Biopsy Issues for Bone and Cartilage Tumors	p. 129
Tissue Harvesting and Fixation	p. 143
Common Fixatives in Hard-Tissue Histology	p. 159
Decalcification of Bone Tissue	p. 167
Principles of Embedding and Common Protocols	p. 185
Infiltration Techniques and Results in Different Types of Resin	p. 199
Sectioning Techniques
Histological Techniques for Decalcified Bone and Cartilage	p. 209
Techniques for Sectioning Undecalcified Bone Tissue Using Microtomes	p. 221
Cutting and Grinding Methods for Hard-Tissue Histology	p. 233
Bone Sectioning Using the Exakt System	p. 243
Bone Sectioning Using a Modified Inner Diamond Saw	p. 253
Bone Sectioning Using the Precise 1 Automated Cutting System	p. 265
Bone, Cement, and Metal Implant Interface Preparation with a High-Pressure Water Cutter	p. 271
Staining Techniques
Basic Staining and Histochemical Techniques and Immunohistochemical Localizations Using Bone Sections	p. 281
Basic Staining Techniques for Cartilage Sections	p. 287
Histochemical and Immunohistochemical Staining of Cartilage Sections	p. 295
Staining Techniques for Plastic-Embedded Specimens	p. 315
In Situ Hybridization of Bone and Cartilage	p. 321
Analysis Techniques
Bone Histomorphometry: Concepts and Common Techniques	p. 331
Histological Analysis of Bone-Implant Interface	p. 353
Histomorphometric Analysis of Bone-Cement and Cement-Metal Interface	p. 361
Histologic Analysis of Bone Healing	p. 375
Histomorphometry of Metabolic Bone Conditions	p. 391
Histology of Articular Cartilage Repair	p. 411
Histological Analysis of Cartilage Conditions	p. 423
Histological Analysis of Soft-Tissues Biomaterial Interface: Relevance to Dental Implants	p. 439
Pathological Diagnosis of Common Tumors of Bone and Cartilage	p. 447
Electron Microscopy and Radiography
Methods for Transmission and Scanning Electron Microscopy of Bone and Cartilage	p. 497
SEM Methods for Observation of the Bone-Implant Interface	p. 505
TEM Methods for Observation of the Bone-Implant Interface	p. 513
Radiological Examination of Calcified Tissues with Emphasis on Bone	p. 531
Microangiography, Macrosectioning, and Preparation for Contact Radiography	p. 549
Recognizing and Avoiding Artifacts
Recognizing and Interpreting Histology Artifacts in Hard-Tissue Research	p. 561
Index	p. 571
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