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9781574445541

Handbook of HPLC, Second Edition

by ;
  • ISBN13:

    9781574445541

  • ISBN10:

    1574445545

  • Edition: 2nd
  • Format: Hardcover
  • Copyright: 2010-07-14
  • Publisher: CRC Press

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Summary

Now in its second edition, this updated text examines the new advances made in this field since the publication of the first edition twelve years ago. New concepts presented include monolithic columns, bonded stationary phases, micro-HPLC, two-dimensional comprehensive liquid chromatography, gradient elution mode, retention models for ions, and capillary electromigration techniques. The book also addresses HPLC detectors, LC-MS interfaces, nonlinear chromatography, displacement chromatography of peptides and proteins, polymer HPLC, field-flow fractionation, affinity and ion chromatography. Significant areas of HPLC application are also explored, including chiral pharmaceutical, environmental, food, and forensic analysis. Explanatory figures, tables, and extensive references supplement the text.

Table of Contents

Prefacep. xiii
Editorp. xv
Contributorsp. xvii
Fundamentals
Monolithic Stationary Phases in HPLCp. 3
Bonded Stationary Phasesp. 47
Micro-HPLCp. 77
Two-Dimensional Comprehensive Liquid Chromatographyp. 101
Gradient Elution Modep. 119
Capillary Electromigration Techniquesp. 155
HPLC Detectorsp. 207
LC-MS Interfaces: State of the Art and Emerging Techniquesp. 233
Control and Effects of Temperature in Analytical HPLCp. 257
Nonlinear Liquid Chromatographyp. 277
Displacement Chromatography in the Separation and Characterization of Proteins and Peptidesp. 309
Field-Flow Fractionationp. 329
Affinity Chromatographyp. 361
Ion Chromatography: Modes for Metal Ions Analysisp. 385
Retention Models for Ions in HPLCp. 417
Polymer HPLCp. 447
Applications
HPLC in Chiral Pharmaceutical Analysisp. 507
HPLC in Environmental Analysisp. 535
HPLC in Food Analysisp. 561
HPLC in Forensic Sciencesp. 661
Indexp. 683
Table of Contents provided by Ingram. All Rights Reserved.

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