Handbook of Laboratory Animal Management and Welfare

by Wolfensohn, Sarah; Lloyd, Maggie

• ISBN13: 9781405111591
• ISBN10: 1405111593
• Edition: 3rd
• Format: Paperback
• Copyright: 2003-11-17
• Publisher: Wiley-Blackwell

Summary

This third edition of the bestseller Handbook of Laboratory Animal Management & Welfare has been fully revised and updated making it an essential handbook for all those working with animals under the Animals (Scientific Procedures) Act, 1986. Designed to be a practical reference book, it covers every area of animal care and aims to improve animal welfare through best practice and refinement of techniques. It covers principles applicable to all species, including training of licensees, the regulatory framework, euthanasia, management of pain and stress, anaesthesia and surgery, and also includes specific sections on biology and husbandry.

Author Biography

Sarah Wolfensohn and Maggie Lloyd

Table of Contents

Acknowledgements	xii
SECTION 1: GENERAL
1 Education and training for the licence holder	3	(12)
Introduction	3	(1)
Attitudes	3	(1)
Training	4	(3)
Core competencies of personal licence holders	7	(1)
Further information	8	(2)
Websites	10	(5)
2 The regulatory framework	15	(26)
A brief history of the use of animals in research	15	(1)
Legislation and animal use	16	(2)
The Animals (Scientific Procedures) Act 1986	18	(15)
Communication	33	(1)
The ethics of using animals in experiments	33	(4)
Statistics of animal usage	37	(1)
Legislation controlling use of animals in experiments in other countries	37	(2)
Other relevant legislation in the UK	39	(1)
Further information	39	(2)
3 Health, safety and security	41	(18)
Legal framework	41	(4)
The hazards	45	(9)
Miscellaneous health and safety hazards and risks	54	(2)
Lone working	56	(1)
Employee security	56	(1)
Summary	57	(1)
Further information	57	(2)
4 Pain, stress and humane end points	59	(15)
What is stress?	59	(1)
Assessment of pain and distress	60	(1)
Quantification of pain and distress	61	(2)
Defining the humane end point	63	(2)
Development of distress scoring systems	65	(7)
Further information	72	(2)
5 Humane methods of killing	74	(11)
Introduction	74	(2)
Preparation for humane killing	76	(1)
Schedule 1 methods	77	(4)
Other methods	81	(1)
Confirmation of death and disposal of carcasses	82	(1)
Choice of methods	82	(2)
Further information	84	(1)
6 Introduction to laboratory animal husbandry	85	(22)
Selection of animals	85	(2)
Health monitoring	87	(7)
Supply of laboratory animals	94	(1)
Transport of laboratory animals	95	(1)
Handling laboratory animals	96	(1)
Introduction to laboratory animal husbandry	97	(8)
Further information	105	(1)
Websites	106	(1)
7 Anaesthesia of laboratory animals	107	(31)
Choosing an anaesthetic	107	(2)
Pre-operative care	109	(1)
Pre-anaesthetic drugs	110	(2)
General anaesthesia: inhalation or injection?	112	(1)
General anaesthetic drugs	113	(16)
Anaesthetic management	129	(5)
Muscle relaxation in anaesthesia	134	(2)
A guide to anaesthetics/analgesics and related drugs	136	(1)
Further information	137	(1)
8 Management of pain, suffering, distress and lasting harm	138	(12)
Animal care: management of distress	139	(3)
Management of pain	142	(4)
Post-operative care	146	(1)
Additional considerations in pain management	147	(1)
Further information	148	(2)
9 Conduct of minor procedures	150	(32)
Administration of substances	150	(8)
Legal categories and restrictions governing the use of drugs used in experimental animals	158	(1)
Removal of blood	159	(10)
Record keeping	169	(1)
Indwelling cannulae, arterial loops and fistulae	169	(3)
Miscellaneous methods of administration and sampling	172	(3)
Polyclonal antibody production in laboratory animals	175	(3)
Animal models	178	(1)
Equipment for carrying out minor procedures	179	(1)
Further information	180	(2)
10 Introduction to surgery and suturing	182	(31)
Introduction to surgical biology	182	(2)
Principles of surgery	184	(7)
Surgical techniques	191	(3)
Surgical equipment	194	(5)
Perioperative antibiosis and post-operative wound infections	199	(1)
Suturing techniques and materials	200	(11)
Summary	211	(1)
Further information	212	(1)
11 The project licence application	213	(20)
Introduction	213	(2)
The project licence application	215	(12)
The application process	227	(1)
Assessment of the application	228	(1)
Project licence management	229	(1)
Further information	230	(1)
Websites	230	(3)
SECTION 2: SPECIES
For each species covers:
Behaviour
Housing
Feeding
Water
Environment
Breeding
Growth
Handling
Pain and stress recognition
Common diseases and health monitoring
Biological data and useful reference data
Drug doses for anaesthesia
Drug doses for analgesia Growth
12 Small laboratory animals	233	(39)
Rodents	233	(1)
Mouse	233	(8)
Rat	241	(7)
Hamster	248	(4)
Gerbil	252	(4)
Guinea pig	256	(6)
Rabbit	262	(8)
Further information	270	(2)
13 Genetically modified animals and harmful mutants	272	(9)
Introduction	272	(1)
Creation of genetically altered animals	273	(2)
Legislative requirements	275	(1)
Monitoring genetically modified and harmful mutant animals	276	(2)
Management of genetically modified and harmful mutant animals	278	(1)
Summary	279	(1)
Further information	279	(2)
14 Carnivores	281	(23)
Dog	281	(9)
Cat	290	(8)
Ferret	298	(5)
Further information	303	(1)
15 Primates	304	(22)
Supply of non-human primates	304	(2)
Psychological well-being	306	(1)
Nutrition	307	(1)
New World monkeys	308	(2)
Old World monkeys	310	(5)
Handling primates	315	(3)
Pain and stress recognition	318	(2)
Common diseases and health monitoring	320	(2)
Doses of drugs for anaesthesia in primates	322	(2)
Further information	324	(1)
Website	325	(1)
16 The larger domestic species	326	(39)
Supply	326	(1)
Pigs	327	(10)
Ruminants	337	(4)
Sheep	341	(7)
Goats	348	(5)
Cattle	353	(5)
Equines	358	(6)
Further information	364	(1)
17 Birds	365	(15)
Introduction	365	(1)
Anatomical features	365	(3)
Physiological features	368	(1)
Behavior	369	(1)
Housing and husbandry	369	(1)
Feeding	370	(1)
Reproduction	370	(1)
Handling	370	(2)
Pain and stress recognition	372	(1)
Common diseases and health monitoring	372	(1)
Anaesthesia	373	(2)
Post-operative care	375	(1)
Analgesics	376	(2)
Useful data	378	(1)
Further information	379	(1)
Website	379	(1)
18 Amphibia	380	(12)
Introduction	380	(1)
Anurans	381	(5)
Caudata (urodeles)	386	(3)
Handling	389	(1)
Anaesthesia	389	(1)
Techniques	390	(1)
Further information	391	(1)
Websites	391	(1)
19 Wild animals	392	(12)
General considerations	392	(2)
Considerations under the Animals (Scientific Procedures) Act 1986	394	(2)
Health and safety considerations	396	(1)
Identification of wild animals	396	(1)
Anaesthesia of wild animals	396	(6)
Further information	402	(1)
Websites	403	(1)
Glossary	404	(5)
Index	409