Handbook of Phycological Methods: Developmental and Cytological Methods

by Edited by Elisabeth Gantt

• ISBN13: 9780521056632
• ISBN10: 0521056632
• Format: Paperback
• Copyright: 2008-03-24
• Publisher: Cambridge University Press

Summary

The contributors include some of the most eminent specialists in the field of phycology. This volume focuses on cytological and developmental methods, each method already has been applied successfully to algae, and practical examples are given.

Table of Contents

Editor's preface

Introduction

Experimental Algal Systems and Techniques

Control of morphogenesis in Micrasterias

Control of development in Scenedesmus

Mating induction in Oedogonium

Gamete induction and flagellar adhesion in Chlamydomonas reinhardi

Acetabularia: techniques for study of nucleo-cytoplasmic interrelationships

Gamete release, fertilization, and embryogenesis in the Fucales

Hybridization and genetics of brown algae

Development in red algae: elongation and cell fusion

Production and selection of mutants in blue-green algae

Euglena: mutations, chloroplast 'bleaching', and differentiation

Crpthecodinium: sexual reproduction and mutagenesis

Protoplast and spheroplast production

Cytoplasts from coenocyic algal cells

Cytoplasmic streaming and membrane phenomena in cells of Characeae

Microbeam irradiation in Mougeotia

Phototropism: determination of an action spectrum in a tip-growing cell

Chromatic adaption in Fremyella diplosiphon and morphogenetic changes

How to detect the presence of a circadian rhythm

Light and Electron Microscopy: Preparative Methods

Photomicrography and special microscopic techniques

Polarized light, interference, and differential interference (Nomarski) optics

Preparation of algae for light microscopy Margaret

Fixation, embedding, sectioning, and staining of algae for electron microscopy Bernhard

Cytochemical localization

Quantitative cytochemical measurement of DNA in Eudorina (Chlorophyceae)

Autoradiography for light and electron microscopy

Immunochemistry: labeled antibodies

Freeze-fracture and freeze-etch techniques

Preparation of algae for scanning electron microscopy

Replica production and negative staining

Preparation of shadow-cast whole mounts

Stereology: quantitative electron microscopic analysis

Appendixes

Culture collections

List of suppliers

Indexes

Subject

Author

Taxonomic

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