The Handbook of Plant Mutation Screening Mining of Natural and Induced Alleles

by Meksem, Khalid; Kahl, Guenter

• ISBN13: 9783527326044
• ISBN10: 3527326049
• Edition: 1st
• Format: Hardcover
• Copyright: 2010-03-22
• Publisher: Wiley-Blackwell

Summary

Induced mutagenesis is a common and promising method for screening for new crops with improved properties. This title introduces the different methods and then focuses on the screening, detection and analysis of the novel mutations. Written by a global team of authors the book is an indispensable tool for all scientists working on crop breeding in industry and academia.

Author Biography

G?nter Kahl is Professor for Plant Molecular Biology in Frankfurt am Main, Germany. He has served in expert missions for IAEA, FAO and UNESCO across the world and is author of more than 250 scientific publications, including several Wiley-VCH books.

Khalid Meksem is Professor at the Department of Plant, Soil & General Agriculture of Southern Illinois University. He is associate editor of the Journal of Biomedicine and Biotechnology and chaired the Functional Genomic Workshop at the International Plant & Animal Genome Conference in San Diego. He is reviewer for several granting agencies, including the National Science Foundation and the US Department of Agriculture.

Table of Contents

Induced Mutations

Physically induced mutagenesis: Ion beam mutagenesis

Ds transposon mutant lines for saturation mutagenesis of the Arabidopsis genome

Use of mutants from T-DNA insertion populations generated by high-throughput screening

Making mutations is an active process: Examining DNA polymerase errors

Tnt1 induced mutations in Medicago: Characterisation and Applications

Mutation Discovery

Sequencing by synthesis: Mutation detection with the Illumina Genome Analyzer

Chemical methods for mutation detection: The chemical cleavage of mismatch (CCM) method

Mutation detection in plants by enzymatic mismatch cleavage

Mutation scanning and genotyping in plants by high resolution DNA melting

In silico methods: Mutation detection software for Sanger sequencing, fragment and genome analysis

High-Throughput Screening Methods

Use of TILLING for reverse and forward genetics of rice

Sequencing-based high-throughput mutation screening using KeyPointTM technology

Applications in Plant Breeding

Natural and induced mutants of barley: SNPs in genes important for breeding

Association mapping for the exploration of genetic diversity and identification of useful loci for plant breeding

Using mutations in corn breeding programs

Gene targeting as a precise tool for plant mutagenesis

Emerging Technologies

Direct single-molecule DNA sequencing:Helicos BioSciences technology and applications

Mutation detection by genome sequencing with universal probes

Emerging Technologies: Detection of mutations with the Pinpoint Sequencer

Emerging Technologies: Nanopore Sequencing for Mutation Detection

Glossary

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