Hepatitis B and d Protocols

by Hamatake, Robert Kiyoshi; Lau, Johnson Y. N.; Block, Timothy M., Ph.D.; Blumberg, Baruch S.

• ISBN13: 9781588291059
• ISBN10: 1588291057
• Format: Hardcover
• Copyright: 2004-02-01
• Publisher: Humana Pr Inc

Summary

For volume 1: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this first of two volumes Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

Table of Contents

Dedication	v
Foreword	vii
Timothy M. Block
Baruch S. Blumberg
Foreword	ix
T. Jake Liang
Preface	xi
Contributors	xvii
Contents for Volume 2	xxi
Part I. Detection of Viral Markers
Overview of Commercial HBV Assay Systems	3	(12)
Stefan Zeuzem
Detection of HBV DNA in Serum Using a PCR-Based Assay	15	(6)
Hau Tim Chung
Detection of HBV DNA by Oligonucleotide Probing	21	(8)
Hsiang Ju Lin
Detection of HBV RNA in Serum of Patients	29	(12)
Wei Zhang
Hans Jorg Hacker
Maria Mildenberger
Qin Su
Claus H. Schroder
Quantification of HBV Covalently Closed Circular DNA from Liver Tissue by Real-Time PCR	41	(10)
Scott Bowden
Kathy Jackson
Margaret Littlejohn
Stephen Locarnini
In Situ Hybridization for the Detection and Localization of HBV DNA in Liver Sections	51	(6)
Vicky C. H. Lai
Johnson Y. N. Lau
Quantitative Assay of Hepatitis B Surface Antigen in Serum or Plasma Using Laurell Electrophoresis	57	(8)
Wolfram H. Gerlich
Ulrike Wend
Dieter Glebe
In Situ Detection of Hepatitis B Viral Antigens: An Immunohistochemical Approach	65	(6)
Jane W. S. Fang
Johnson Y. N. Lau
Detection of Hepatitis B Virus X Antigen by Immunohistochemistry and Western Blotting	71	(14)
Jie Liu
Mark A. Feitelson
Detection of Serum HDV RNA by RT-PCR	85	(10)
Antonina Smedile
Maria Grazia Niro
Mario Rizzetto
Nonradioisotopic In Situ Hybridization for HDV RNA	95	(4)
Francesco Negro
Detection and Characterization of Small and Large HDV Antigens	99	(8)
Thomas B. Macnaughton
Michael M. C. Lai
Immunohistochemical Detection of Hepatitis Delta Antigen	107	(8)
Karim Abid
Francesco Negro
Part II. Viral Genotypes and Variants
HBV Vaccine-Escape Variants	115	(10)
Ashraf A. Basuni
William F. Carman
HBV Genotyping and Analysis for Unique Mutations	125	(26)
Anna Ayres
Stephen Locarnini
Angeline Bartholomeusz
A One-Filter-Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus	151	(14)
Chiaho Shih
Ta-Tung Thomas Yuan
Detection of Hypermodified Middle-Envelope (M) Proteins Secreted from Naturally Occurring HBV Variants Containing a preS2 Internal Deletion	165	(10)
Chiaho Shih
Pei-Ching Tai
Hepatitis B Viral Genotyping with the Research INNO-LiPA HBV Genotyping Line Probe Assay	175	(14)
Sija De Gendt
Fred Shapiro
Jelena Juras
Els Van Assche
Geert Maertens
Erwin Sablon
Part III. Molecular Biological Characterization
Processing of Hepatitis B Virus Surface Proteins	189	(10)
Volker Bruss
Binding of Duck Carboxypeptidase D to Duck Hepatitis B Virus	199	(14)
Stephan Urban
Nucleoprotein Transport of HBV Capsid Particles	213	(14)
Michael Kann
Phosphorylation Analysis of Hepatitis B Virus Core Protein in Mammalian Cells	227	(8)
Jie Li
Yanyan Zheng
Jinah Choi
Jing-hsiung Ou
Study of HBV Replication Capacity in Relation to Sequence Variation in the Precore and Core Promoter Regions	235	(12)
Fabien Zoulim
ShuPing Tong
Christian Trepo
A cis/trans Genetic Test for Pleiotropic Phenotypes Associated with a Frequent Naturally Occurring Mutation at Amino Acid 97 of HBV Core Protein	247	(12)
Chiaho Shih
Ta-Tung Thomas Yuan
Studying DHBV Polymerase by In Vitro Transcription and Translation	259	(12)
Jianming Hu
Expression and Purification of Functional Hepatitis B Virus Polymerase in the Baculovirus Insect Cell System	271	(10)
Lisa Lott
Lena Notvall
Robert E. Lanford
Localization of Duck Hepatitis B Virus Polymerase Within Cells	281	(14)
Ermei Yao
John E. Tavis
Endogenous Polymerase Assay for the Analysis of Hepatitis B Virus in Transgenic Mice	295	(8)
Zhenming Xu
Jing-hsiung Ou
Transcriptional Control of Hepatitis B Virus	303	(12)
Anneke K. Raney
In Vitro Reconstitution of ε-Dependent Duck Hepatitis B Virus Replication Initiation	315	(12)
Jurgen Beck
Michael Nassal
Hepatitis B Viruses: A Triple Threat for Malignant Transformation of Hepatocytes	327	(12)
Charles E. Rogler
Index	339