Hepatitis B and d Protocols

by Hamatake, Robert Kiyoshi; Lau, Johnson Y. N.

• ISBN13: 9781588291080
• ISBN10: 1588291081
• Format: Hardcover
• Copyright: 2004-02-01
• Publisher: Humana Pr Inc

Summary

For volume 2: A broad-ranging collection of cutting-edge techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this second of two volumes, Immunology, Model Systems, and Clinical Studies, the authors focus on protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying first volume, Detection, Genotypes, and Characterization, contains user-friendly protocols for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

Table of Contents

Dedication	v
Foreword by Timothy M. Block and Baruch S. Blumberg	vii
Foreword by T. Jake Liang	ix
Preface	xi
Contributors	xvii
Contents for Volume 1	xxi
PART I. VIRAL-SPECIFIC IMMUNOLOGICAL AND OTHER HOST RESPONSES
1 Studying Host Immune Responses Against Duck Hepatitis B Virus Infection
Darren S. Miller, Edward M. Bertram, Catherine A. Scougall, Ieva Kotlarski, and Allison R. Jilbert	3	(24)
2 Measurement of Cell-Mediated Immune Response In Woodchucks
Stephan Menne and Paul J. Cote	27	(10)
3 Study of Liver-Specific Expression of Cytokines During Woodchuck Hepatitis Virus Infection
Mengji Lu and Michael Roggendorf	37	(6)
4 Induction of Anti-Hepatitis B Virus Immune Responses Through DNA Immunization
Michael Geissler, Robert Weth, Christian F. Grimm, Dorte Ortmann, and Hubert E. Blum	43	(12)
5 Monitoring Gene Expression Using DNA Microarrays During Hepatitis B Virus Infection
Michael R. Beard and Stephen Locarnini	55	(10)
6 Determination of Hepatitis B Virus-Specific CD8+ T-Cell Activity in the Liver
Michelins Nascimbeni and Barbara Rehermann	65	(20)
7 Determining the Precursor Frequency of HBV Nucleocapsid Antigen-Specific T Cells
Jennifer A. Waters and Howard C. Thomas	85	(4)
8 Detection and Characterization of Virus-Specific CD8+T Cells Using the Tetramer Approach
Xiao-Song He and Harry B. Greenberg	89	(8)
9 In Vitro Analysis of Hepatitis B Virus Specific CD4+ T Cells
Shilpa Chokshi and Nikolai V. Naoumov	97	(14)
10 Induction of Humoral and Cellular Immune Responses to Hepatitis Delta Virus Through DNA Immunization in BALB/c Mice
Ren-Shiang Lee, Shih-Jer Hsu, Li-Rung Huang, Hui-Lin Wu, Shiou-Lin Lin, Ding-Shinn Chen, and Pei-Jer Chen	111	(20)
PART II. IN VITRO AND IN VIVO MODELS
11 Infection of Primary Chimpanzee Hepatocytes with Recombinant Hepatitis D Virus Particles: A Surrogate Model for Hepatitis B Virus
Azeneth Barrera and Robert E. Lanford	131	(12)
12 Study of the Endocytosis and Intracellular Localization of Subviral Particles of Hepatitis B Virus in Primary Hepatocytes
Dieter Glebe and Wolfram H. Geruch	143	(10)
13 The Tupaia Model for the Study of Hepatitis B Virus: Direct Infection and HBV Genome Transduction of Primary Tupaia Hepatocytes
Fritz von Weizsacker, Josef Kock, Sabine MacNelly, Shaotang Ren, Hubert F. Blum, and Michael Nassal	153	(10)
14 Delivery of Hepatitis B Virus Therapeutic Agents Using Asialoglycoprotein Receptor-Based Liver-Specific Targeting
Masayoshi Konishi, Catherine H. Wu, and George Y. Wu	163	(12)
15 Woodchuck Hepatitis Virus Hepatocyte Culture Models
Norma D. Churchill and Tomasz I. Michalak	175	(14)
16 Duck Hepatitis B Virus Primary Hepatocyte Culture Model
Olivier Hantz and Fabien Zoulim	189	(10)
17 Enhancement of Infection of HepG2 Cells in Culture by Predigestion of Hepadnavirus with V8 Protease
Xuanyong Lu and Timothy M. Block	199	(10)
18 Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus
Martin Sprinzl, Jerome Dumortier, and Ulrike Protzer	209	(10)
19 Baculovirus-Mediated Gene Transfer for the Study of Hepatitis B Virus
Harriet C. Isom, Ayman M. Abdelhamed, John P. Bilello, and Thomas G. Miller	219	(20)
20 Transgenic Hepatitis B Virus Mouse Model In the Study of Chemotherapy
John D. Morrey	239	(14)
21 Transplantation of Human Hepatocytes in Immunodeficient UPA Mice: A Model for the Study of Hepatitis B Virus
Jorg Petersen, Martin R. Burda, Maura Dandri, and Charles E. Rogler	253	(8)
22 Duck Hepatitis B Virus Model in the Study of Hepatitis B Virus
Lucyna Cova and Fabien Zoulim	261	(8)
23 Hepatitis B Virus Transgenic Severe Combined Immunodeficient Mouse Model of Acute and Chronic Liver Disease
Mark A. Feitelson, Jonathan D. Larkin, and Raymond F. Schinazi	269	(20)
24 The Chimpanzee Model: Contributions and Considerations for Studies of Hepatitis B Virus
Pascal Gagneux and Elaine A. Muchmore	289	(30)
25 Hepatitis B in Liver Transplant Recipients as a Special Model of Antiviral Drug Development
Robert G. Gish and Adil Wakil	319	(24)
26 Hepatitis Delta Virus Transfection for the Mouse In Vivo Model
Jinhong Chang, Anthony Lerro, and John M. Taylor	343	(8)
27 Hepatitis Delta Virus RNA Transfection for the Cell Culture Model
Thomas B. Macnaughton and Michael M.C. Lai	351	(10)
PART III. ANTIVIRAL TESTING AND CLINICAL STUDIES
28 Analysis of Hepatitis B Virus Dynamics and Its Impact on Antiviral Development
Manuel Tsiang and Craig S. Gibbs	361	(18)
29 Genotyping Anti-Hepatitis B Virus Drug Resistance
Eric J. Bourne, Josee Gauthier, Vincent A. Lopez, and Lynn D. Condreay	379	(8)
30 A Parsimonious Method for Screening Drug Combinations for Antihepadnaviral Activity Using a Parametric Dose-Response Surface Approach
Tim Shaw and Stephen Locarnini	387	(28)
31 Hepatitis B Virus: Where Are We and Where Are We Going?
Michael P. Manns, Johannes Hadem, and Heiner Wedemeyer	415	(30)
32 Designing Studies to Evaluate Anti-Hepatitis B Virus Therapies: From the Perspective of Studies for the Registration of Pharmaceutical Products
Alison B. Murray	445	(12)
33 Specific Considerations In the Design of Hepatitis B Virus Clinical Studies in the Far East
Man-Fung Yuen and Ching-Lung Lai	457	(8)
34 Studying the Treatment of Chronic Hepatitis B Viral Infection in Special Populations
Robert G. Gish and Stephen Locarnini	465	(34)
35 Designing Clinical Development Programs for Anti-Hepatitis B Virus Drugs
Nathaniel A. Brown	499	(40)
36 Testing Antivirals Against Hepatitis Delta Virus: Farnesyl Transferase Inhibitors
Bruno B. Bordier and Jeffrey S. Glenn	539	(16)
Index	555