Hepatocyte Transplantation

by Dhawan, Anil; Hughes, Robin D.

• ISBN13: 9781588298836
• ISBN10: 1588298833
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-02-28
• Publisher: Humana Pr Inc

Summary

Due to its efficacy in animal models, cellular therapy using human hepatocytes is being evaluated worldwide as an alternative to organ transplantation in patients with liver-based metabolic disease and acute liver failure. In Hepatocyte Transplantation: Methods and Protocols, an international panel of experts provide up-to-date laboratory and clinical techniques covering the many key areas necessary for successful transplantation, such as cryopreservation, quality assurance, detection of cell engraftment, and the future of the field with the development of foetal hepatoblasts and stem cell derived hepatocytes. Written in the highly successful Methods in Molecular Biology'„¢ series format, the chapters in this volume present brief introductions to the material, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Hepatocyte Transplantation: Methods and Protocols is an ideal guide for researchers setting out in the rapidly progressing field of hepatocyte transplantation, as well as those who already have experience with this new therapy for liver disease.

Table of Contents

Human Hepatocyte Transplantation Overview

Isolation of Human Hepatocytes

An Optimised Method for Cryopreservation of Human Hepatocytes

Liver Cell Culture Techniques

In vitro Assays for Induction of Drug Metabolism

Hepatocyte ApoptosisMustapha Najimi

Small Animal Models of Hepatocyte TransplantationJurgen Seppen

Hepatocyte Transplantation Techniques: Large Animal ModelsAnne Weber

Cell Transplant Techniques: Engraftment Detection of Cells

Hepatic Preconditioning for Transplanted Cell Engraftment and Proliferation

Ex vivo Gene Transfer into Hepatocytes

Sources of Adult Hepatic Stem Cells: Haematopoietic

Production of Hepatocyte-like Cells from Human Amnion

Generation of Hepatocytes from Human Embryonic Stem Cells

Isolation, In vitro Cultivation and Characterisation of Foetal Liver Cells

Human Intrahepatic Biliary Epithelial Cell Lineages: Studies in vitro

Liver Cell Labelling with MRI Contrast Agents Michel Modo

Microbiological Monitoring of Hepatocyte Isolation in the GMP Laboratory

Table of Contents provided by Publisher. All Rights Reserved.