High Content Screening

by Taylor, D. Lansing; Haskins, Jeffrey R.; Giuliano, Kenneth A.

• ISBN13: 9781588297310
• ISBN10: 1588297314
• Edition: CD
• Format: Hardcover
• Copyright: 2006-10-31
• Publisher: Humana Pr Inc

Summary

High content screening (HCS) was developed by Cellomics Inc. in the mid-1990s to address the need for a platform that could be used in the discovery-driven research and development required to understand the functions of genes and gene products at the level of the cell. High Content Screening: A Powerful Approach to Systems Cell Biology and Drug Discovery discusses its use as a high throughput platform to understand the functions of genes, RNA, proteins, and other cellular constituents at the level of the living cell. High Content Screening is assembled to assist both existing users of HCS, as well as investigators considering the addition of a discovery-driven platform to their research and development activities. The chapters have been organized into sections that highlight the importance of integrating instrumentation, application software, reagents, and informatics. In addition, there is a combination of pure review chapters on key topics and specific methods chapters. Written by a team of world leaders in the field, High Content Screening is essential reading for senior scientists, advanced lab technicians, as well as students/post-docs who wish to apply HCS in their research and development programs.

Table of Contents

Preface	v
Contributors	xi
Guide to the Companion CD	xiii
Part I. Introduction to High Content Screening
Past, Present, and Future of High Content Screening and the Field of Cellomics	3	(16)
D. Lansing Taylor
A Pharmaceutical Company User's Perspective on the Potential of High Content Screening in Drug Discovery	19	(14)
Ann F. Hoffman
Ralph J. Garippa
Linking Microscopy and High Content Screening in Large-Scale Biomedical Research	33	(8)
James G. Evans
Paul Matsudaira
Part II. Instrumentation, Biological Application Software, and Sample Preparation
Requirements, Features, and Performance of High Content Screening Platforms	41	(22)
Albert H. Gough
Paul A. Johnston
Characteristics and Value of Directed Algorithms in High Content Screening	63	(20)
Richik N. Ghosh
Oleg Lapets
Jeffrey R. Haskins
Characteristics and Value of Machine Learning for Imaging in High Content Screening	83	(12)
Juergen A. Klenk
Tools for Quantitative and Validated Measurements of Cells	95	(14)
Anne L. Plant
John T. Elliott
Alessandro Tona
Dennis McDaniel
Kurt J. Langenbach
Automated Cell Plating and Sample Treatments for Fixed Cells in High Content Assays	109	(12)
Gillian R. Richards
Julie E. Kerby
Grace K. Y. Chan
Peter B. Simpson
Differentiating Primary Human Cells in Rapid-Throughput Discovery Applications	121	(8)
Daniel R. Marshak
Dale E. Greenwalt
Use of the CellCard™ System for Analyzing Multiple Cell Types in Parallel	129	(12)
Oren Beske
Daniel Bassoni
Simon Goldbard
Part III. Reagents
Reagents to Measure and Manipulate Cell Functions	141	(24)
Kenneth A. Giuliano
D. Lansing Taylor
Alan S. Waggoner
Fluorescent Proteins and Engineered Cell Lines	165	(24)
Nick Thomas
Optimizing the Integration of Immunoreagents and Fluorescent Probes for Multiplexed High Content Screening Assays	189	(6)
Kenneth A. Giuliano
The HaloTag™: A Novel Technology for Cell Imaging and Protein Analysis	195	(14)
Georgyi V. Los
Keith Wood
Protein Labeling With FlAsH and ReAsH	209	(12)
Thomas Machleidt
Matt Robers
George T. Hanson
Exploiting Network Biology to Improve Drug Discovery	221	(12)
Marnie L. MacDonald
John K. Westwick
Physiocological Indicators of Cell Function	233	(12)
Michael J. Ignatius
Jeffrey T. Hung
The Use of siRNA to Validate Immunofluorescence Studies	245	(8)
K. Gregory Moore
Wayne Speckmann
Ronald P. Herzig
Caged Substrates Applied to High Content Screening: An Introduction With an Eye to the Future	253	(16)
Peter G. Conrad, II
Rajesh V. Chavli
Richard S. Givens
Part IV. Informatics and Bioinformatics
Overview of Informatics for High Content Screening	269	(12)
R. Terry Dunlay
Wallace J. Czekalski
Mark A. Collins
Large-Scale Data Management for High Content Screening	281	(12)
Leon S. Garfinkel
An Integrated Biomedical Knowledge Extraction and Analysis Platform: Using Federated Search and Document Clustering Technology	293	(8)
Donald P. Taylor
Visualization of High Content Screening Data	301	(18)
Matthew J. Anstett
Pathway Mapping Tools for Analysis of High Content Data Sean	319	(34)
Ekins
Yuri Nikolsky
Andrej Bugrim
Eugene Kirillov
Tatiana Nikolskaya
Part V. Assays and Applications of High Content Screening
Systems Biology in Cancer Research: Genomics to Cellomics	353	(14)
Jackie L. Stilwell
Yinghui Guan
Richard M. Neve
Joe W. Gray
Target Validation in Drug Discovery	367	(12)
Robert A. Blake
High Content Screening as a Screening Tool in Drug Discovery	379	(10)
Anthony Nichols
Discovery of Protein Kinase Phosphatase Inhibitors	389	(12)
Andreas Vogt
John S. Lazo
High Content Translocation Assays for Pathway Profiling	401	(14)
Frosty Loechel
Sara Bjorn
Viggo Linde
Morten Praestegaard
Len Pagliaro
In Vitro Cytotoxicity Assessment	415	(12)
Peter O'Brien
Jeffrey Haskins
Neurite Outgrowth in Retinal Ganglion Cell Culture	427	(8)
John B. Kerrison
Donald J. Zack
Index	435