High Content Screening: Science,... | Buy

The authoritative reference on High Content Screening (HCS) in biological and pharmaceutical research, this guide covers: the basics of HCS: examples of HCS used in biological applications and early drug discovery, emphasizing oncology and neuroscience; the use of HCS across the drug development pipeline; and data management, data analysis, and systems biology, with guidelines for using large datasets. With an accompanying CD-ROM, this is the premier reference on HCS for researchers, lab managers, and graduate students.

Preface
Contributors
Essentials of High Content Screening
Approaching High Content Screening and Analysis: Practical Advice for Users
Introduction
What is HCS and Why Should I Care?
How does HCS Compare with Current Assay Methods?
The Basic Requirements to implement HCS
The Process
An Example Approach
Six Considerations for HCS Assays
References
Automated High Content Screening Microscopy
Introduction
Automated HCS Imaging Requirements
Components of Automated Imaging Platforms
Imaging Platform Software
Data Storage and Management
Selecting an HCS Platform
Comparison of a SAPK Activation HCS Assay Read on an ArrayScanw 3.1, an ArrayScanw VTi, and an IN Cell 3000 Automated Imaging Platform
References
A Primer on Image Informatics of High Content Screening
Background
HCS Image Processing
Validation
Information System Management
Data Modeling
Conclusions
Acknowledgements
References
Developing Robust High Content Assays
Introduction
Overview of a Typical Immunofluorescence-Based High Content Assay
Identifying Sources of Variability in a High Content Assay
From Immunofluorescence to High Content: Selecting the Right Metric
Validation of High Content Assays
Conclusion
Acknowledgements
References
Applications of Hcs In Basic Science and Early Drug Discovery
HCS in Cellular Oncology and Tumor Biology
Cancer Cell Biology and HCS
The Cell Biology of Cell Death
Cell Signaling Pathways in Cancer
HCS in Tumor Biology
Conclusions
References
Exploring the Full Power of Combining High Throughput RNAi with High Content Readouts: From Target Discovery Screens to Drug Modifier Studies
Background: The Convergence of High Content Analysis and RNAi
Integrating HT-RNAi and HCA in Drug Discovery: The Potential
Combining RNAi and HCA in one Assay - the Reality
HCA-Based RNAi Studies - The Future
Acknowledgements
References
Leveraging HCS in Neuroscience Drug Discovery
High Content Screening and Drug Discovery
The Neuron and Neuronal Morphology
Methods for Measuring Neuronal Morphology
Small Molecule Screening for Neurite Outgrowth
RNAi in Neuroscience and HCA&<$$$>
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