Histological Analysis of Endocrine Disruptive Effects in Small Laboratory Fish

by Daniel Dietrich; Heiko O. Krieger

• ISBN13: 9780470431788
• ISBN10: 0470431784
• Format: eBook
• Copyright: 2009-07-01
• Publisher: Wiley-Interscience

Summary

Timely title assembling the combined knowledge of some of the leading authorities in the field of small fish reproduction - an important topic for risk assessment and registration of chemical, agricultural, and pharmaceutical compounds Provides guidance on the microscopic structure of living tissue and evaluation of the reproductive glands of small laboratory fish Includes state-of-the-art science along with sufficient anatomical and physiological background for understanding and interpreting test results Helps standardize the interpretation of results from aquatic bioassays and field observations, which will also clarify inconsistencies in the current scientific literature

Table of Contents

Preface

Acknowledgments

Contributing Authors

Introduction

References

Fish Species of Interest

Fathead Minnow (Pimephales promelas)

Medaka (Oryzias latipes)

Zebrafish (Danio rerio)

Other Fish Species

References

Sexual Determination, Differentiation, and Gonadal Development

Primordial Germ Cells in the Primordial (Primary) Gonad

Reproductive Strategies

Differentiation of the Primordial Gonad into Ovary or Testis

Gonadal Duct Formation

Endocrinology: Influence on Gonadogenesis

Critical Period of Sexual Differentiation in Developing Fish

Bi-Potentiality of Germ Cells in Adult Fish

References

Female Gonad Anatomy and Morphology

Gonadogenesis: Ovary

Hypothalamic-Pituitary-Ovarian Axis

Cellular Structure of the Ovary

References

Male Gonad Anatomy and Morphology

Gonadogenesis: Testes

References

Endocrine-Disrupting Compounds

Individual Effects

Effects Associated with Exposure to Specific Compounds or Compound Classes

Population Effects

References

Determination of Effects of Exogenous Hormones and Endocrine-like Active Compounds

Histological Processing: Microdissection versus Whole-Fish Sectioning

Optimal Tissue Preparation and Histological Techniques

Plane of Gonad Sectioning for Optimal Organ Representation

References

Evaluation of Effects in Fish Gonads

Qualitative (Semiquantitative) versus Quantitative Evaluation

Gonadal Staging in the Testis

Gonadal Staging in the Ovary

Qualitative Assessment of Histopathological Changes

References

Experimental Design and Statistics

Basic Considerations in Experimental Design

Variables to be Determined and Their Inherent Biological and Mathematical Characteristics

Prerequisite Statistical Concepts

Statistical Tests and Testing Situations Encountered Routinely

References

Conclusions

References

Appendix: Fish Preparation and Microdissection of Organs

Fish Preparation

Microdissection of Organs

Tissue Fixation

Embedding

Tissue Sectioning

Sample Mounting

Tissue Slide Staining

Final Processing

Examples

Summary

References

Index

Table of Contents provided by Publisher. All Rights Reserved.