HPLC Made to Measure A Practical Handbook for Optimization

by Kromidas, Stavros

• ISBN13: 9783527313778
• ISBN10: 352731377X
• Edition: 1st
• Format: Hardcover
• Copyright: 2006-07-21
• Publisher: Wiley-VCH

Summary

The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness. This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.

Author Biography

Stavros Kromidas worked as Sales Manager for Waters Chromatography following his PhD thesis completed in 1983. From 1989 to 2001 he was Managing Director of Novia GmbH, a consultant company for analytical chemistry and is an independent consultant since then. He has worked in the area of HPLC since 1978 and has given lectures and training courses since 1984. He is author and co-author of several articles and numerous successful books.

Table of Contents

ESSENTIAL ASPECTS OF OPTIMIZATION

Principles of HPLC Optimization Illustrated by RP-Chromatography

Fast Gradient Separations

pH and Selectivity in Reversed-Phase Chromatography

Selecting the Correct pH-Value for HPLC

Optimization of Data Evaluation in Chromatography

Calibration Characteristics and Uncertainty indicating Starting-Points for Method Optimization

Comparison and Choice of Columns for RP-HPLC

Column Selectivity in Reversed-Phase Chromatography

The Use of Principal Component Analysis for the Characterisation of Reversed-Phase Liquid Chromatographic Stationary Phases

Chemometrics -

A Powerful Tool for Handling a Large Number of Data

Linear Free Energy Relations -

Tools for Column Characterization and Method Optimization in HPLC?

OPTIMIZATION IN DIFFERENT LC-MODI

Column Selectivity in Reversed-Phase Liquid Chromatography

Understanding Selectivity by Use of Suspended-State High Resolution Magic Angle Spinning NMR Spectroscopy

Optimization in Normal-Phase HPLC

Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection

Gel Filtration -

Size Exclusion Chromatography of biopolymers

Optimization in Affinity Chromatography

Optimization of Chiral Separations in HPLC

µLC/nano LC -

Optimization and Troubleshooting

Microchip-Based Liquid Chromatography -

Techniques and Possibilities

Ultra-Performance Liquid Chromatography

HYPHENATED TECHNIQUES

Immunochromatographic Techniques

Enhanced Characterization and Comprehensive Analyses by 2-dimensional Chromatography

LC/MS -

Hints and Recommendations for Optimization and Troubleshooting

LC-NMR-Coupling

COMPUTER-AIDED OPTIMIZATION

Computer-Facilitated HPLC Method Development Using DryLab Software

ChromSword Software for Automated and Computer-Assisted Development of HPLC Methods

Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC

USER REPORTS

NanoLC-MS/MS in Proteomics

Ways for the Verification of the Robustness in RP-HPLC

Separation of Complex Sample Mixtures

Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC-UV/MS/CLND